PMID- 12810255
OWN - NLM
STAT- MEDLINE
DCOM- 20030728
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 144
IP  - 1
DP  - 2003 Jul 1
TI  - Establishment of a new human malignant fibrous histiocytoma cell line, FU-MFH-1: 
      cytogenetic characterization by comparative genomic hybridization and 
      fluorescence in situ hybridization.
PG  - 44-51
AB  - Although a number of malignant fibrous histiocytoma (MFH) cell lines have been 
      reported, their characterization at a molecular cytogenetic level has not been 
      fully established. In this study, we established a new human cell line, 
      designated as FU-MFH-1, from a storiform-pleomorphic MFH arising in the 
      retroperitoneum of a 61-year-old woman, and applied comparative genomic 
      hybridization (CGH) and fluorescence in situ hybridization (FISH) with chromosome 
      painting probes for the characterization of chromosome alterations. FU-MFH-1 
      cells were spindle, round, or polygonal in shape with oval nuclei, and were 
      maintained continuously in vitro for over 50 passages for more than 12 months. 
      G-banding analysis was performed and FU-MFH-1 revealed a complex karyotype with 
      an abnormal chromosome 19 containing a homogeneously staining region (hsr). CGH 
      analysis showed a high-level amplification of 12q13-->q21. The high-level 
      amplification detected by CGH was refined by FISH. These results showed that the 
      hsr was composed of amplified DNA sequences from 12q. Our study emphasizes the 
      usefulness of CGH as a powerful tool for chromosomal localization of amplified 
      sequences. The FU-MFH-1 cell line should be useful for biologic and molecular 
      pathogenetic investigations of human MFH.
FAU - Nishio, Jun
AU  - Nishio J
AD  - Department of Pathology, School of Medicine, Fukuoka University, Fukuoka, Japan. 
      nishio@minf.med.fukuoka-u.ac.jp
FAU - Iwasaki, Hiroshi
AU  - Iwasaki H
FAU - Ishiguro, Masako
AU  - Ishiguro M
FAU - Ohjimi, Yuko
AU  - Ohjimi Y
FAU - Nishimura, Noriko
AU  - Nishimura N
FAU - Koga, Takamasa
AU  - Koga T
FAU - Kawarabayashi, Tatsuhiko
AU  - Kawarabayashi T
FAU - Kaneko, Yasuhiko
AU  - Kaneko Y
FAU - Kikuchi, Masahiro
AU  - Kikuchi M
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Animals
MH  - Female
MH  - Histiocytoma, Benign Fibrous/genetics/*pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Mice
MH  - Mice, SCID
MH  - Middle Aged
MH  - Nucleic Acid Hybridization/*methods
MH  - Tumor Cells, Cultured
EDAT- 2003/06/18 05:00
MHDA- 2003/07/29 05:00
CRDT- 2003/06/18 05:00
PHST- 2003/06/18 05:00 [pubmed]
PHST- 2003/07/29 05:00 [medline]
PHST- 2003/06/18 05:00 [entrez]
AID - S0165460802009251 [pii]
AID - 10.1016/s0165-4608(02)00925-1 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2003 Jul 1;144(1):44-51. doi: 
      10.1016/s0165-4608(02)00925-1.