PMID- 12811506
OWN - NLM
STAT- MEDLINE
DCOM- 20040810
LR  - 20181113
IS  - 0172-8172 (Print)
IS  - 0172-8172 (Linking)
VI  - 24
IP  - 2
DP  - 2004 Mar
TI  - Generation of three-dimensional pannus-like tissues in vitro from single cell 
      suspensions of synovial fluid cells from arthritis patients.
PG  - 71-6
AB  - Using single cell suspensions from synovial fluid cells of arthritis patients, we 
      observed differentiation of three-dimensional tissues in vitro. This new model of 
      pannus-like tissue (PLT) might be useful to study pannus tissue formation and 
      differentiation. In the PLT cultures, we observed two cell types, fibroblast-like 
      and macrophage-like cells, defined by their distinct morphology and major 
      histocompatibility complex (MHC) by human leukocyte antigen (HLA) class II 
      expression. We could discriminate several intermediate steps of differentiation 
      which finally led to 3D villi-like structures. Secretion of interferon gamma, 
      interleukin-10, and tumor necrosis factor alpha was measured in the culture 
      supernatants. Using methotrexate at various concentrations, the growth of PLT 
      could be inhibited. We describe definite intermediate steps of differentiation. 
      The present approach could be a suitable model for the in vitro study of pannus 
      tissue formation.
FAU - Solomon, Samuel
AU  - Solomon S
AD  - Department of Biology, Faculty of Sciences, University of Konstanz, M662, 78457, 
      Konstanz, Germany.
FAU - Masilamani, Madhan
AU  - Masilamani M
FAU - Mohanty, Subhasis
AU  - Mohanty S
FAU - Schwab, Jorg E
AU  - Schwab JE
FAU - Boneberg, Eva-Maria
AU  - Boneberg EM
FAU - Illges, Harald
AU  - Illges H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030617
PL  - Germany
TA  - Rheumatol Int
JT  - Rheumatology international
JID - 8206885
RN  - 0 (Cytokines)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Immunosuppressive Agents)
RN  - YL5FZ2Y5U1 (Methotrexate)
SB  - IM
MH  - Arthritis, Reactive/physiopathology
MH  - Arthritis, Rheumatoid/*physiopathology
MH  - Cell Death/physiology
MH  - Cell Differentiation/drug effects/*physiology
MH  - Cells, Cultured
MH  - Connective Tissue Cells/drug effects/*physiology
MH  - Culture Techniques/methods
MH  - Cytokines/biosynthesis
MH  - Fibroblasts/drug effects/physiology
MH  - Histocompatibility Antigens Class II/biosynthesis
MH  - Humans
MH  - Immunosuppressive Agents/pharmacology
MH  - Macrophages/drug effects/physiology
MH  - Methotrexate/pharmacology
MH  - Synovial Membrane/cytology/*physiopathology
EDAT- 2003/06/18 05:00
MHDA- 2004/08/11 05:00
CRDT- 2003/06/18 05:00
PHST- 2002/02/27 00:00 [received]
PHST- 2003/03/09 00:00 [accepted]
PHST- 2003/06/18 05:00 [pubmed]
PHST- 2004/08/11 05:00 [medline]
PHST- 2003/06/18 05:00 [entrez]
AID - 10.1007/s00296-003-0333-0 [doi]
PST - ppublish
SO  - Rheumatol Int. 2004 Mar;24(2):71-6. doi: 10.1007/s00296-003-0333-0. Epub 2003 Jun 
      17.