PMID- 12812049
OWN - NLM
STAT- MEDLINE
DCOM- 20031212
LR  - 20061115
IS  - 0379-4172 (Print)
IS  - 0379-4172 (Linking)
VI  - 30
IP  - 4
DP  - 2003 Apr
TI  - A chimeric mouse model established by allogenic in utero transplantation.
PG  - 289-94
AB  - In utero stem cells transplantation is a promising approach to the prenatal 
      treatment of diseases. In order to investigate the fate of the stem cells after 
      in utero transplantation, we have established a chimeric mouse model with the 
      method of in utero transplantation. Mononuclear cells (including stem 
      cells/progenitor cells) derived from male mouse bone marrow were injected into 
      fetal mouse peritoneal cavity during the pre-immune period. The donor cells in 
      the circulatory blood of female recipients were identified by fluorescence in 
      situ hybridization (FISH), and the Y-chromosome specific DNA was detected by PCR 
      as well as real-time quantitative PCR after the recipient mice were born. The 
      results showed that a total of 4 female recipient mice were chimeric in their 
      peripheral blood. Significantly, the donor cells in three chimeric mice persisted 
      up to six months.
FAU - Wang, Mo-Lin
AU  - Wang ML
AD  - Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai 
      Communication University, Shanghai 200040, China.
FAU - Yan, Jing-Bin
AU  - Yan JB
FAU - Xiao, Yan-Ping
AU  - Xiao YP
FAU - Ren, Zhao-Rui
AU  - Ren ZR
FAU - Huang, Shu-Zhen
AU  - Huang SZ
FAU - Zeng, Yi-Tao
AU  - Zeng YT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yi Chuan Xue Bao
JT  - Yi chuan xue bao = Acta genetica Sinica
JID - 7900784
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Female
MH  - Fetus/*surgery
MH  - Hematopoietic Stem Cell Transplantation/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Models, Animal
MH  - Pregnancy
MH  - Pregnancy Outcome
MH  - Transplantation Chimera/*blood/genetics
MH  - Transplantation, Homologous
MH  - Weaning
EDAT- 2003/06/19 05:00
MHDA- 2003/12/13 05:00
CRDT- 2003/06/19 05:00
PHST- 2003/06/19 05:00 [pubmed]
PHST- 2003/12/13 05:00 [medline]
PHST- 2003/06/19 05:00 [entrez]
PST - ppublish
SO  - Yi Chuan Xue Bao. 2003 Apr;30(4):289-94.