PMID- 12812463
OWN - NLM
STAT- MEDLINE
DCOM- 20040122
LR  - 20190516
IS  - 1058-0468 (Print)
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Linking)
VI  - 20
IP  - 5
DP  - 2003 May
TI  - High sex chromosome aneuploidy and diploidy rate of epididymal spermatozoa in 
      obstructive azoospermic men.
PG  - 196-203
AB  - PURPOSE: To evaluate the frequencies of sex chromosome aneuploidy and diploidy 
      rate of epididymal spermatozoa from obstructive azoospermic men and its impact on 
      intracytoplasmic sperm injection (ICSI) outcomes. METHODS: Epididymal spermatozoa 
      retrieved from 24 obstructive azoospermic men and ejaculated spermatozoa from 24 
      fertile donors were analyzed using triple color fluorescence in situ 
      hybridization (FISH) techniques, in order to investigate the rates of diploidy 
      and aneuploidy for chromosomes 18, X and Y. RESULTS: Epididymal spermatozoa from 
      obstructive azoospermic men had total sex aneuploidy, disomy 18, and diploidy 
      rates significantly higher than ejaculated spermatozoa from normozoospermic 
      fertile controls (1.44% vs 0.14%, 0.11% vs 0.02%, and 0.18% vs 0.02%, 
      respectively; p < 0.005). There were no statistically significant differences in 
      ICSI outcomes between the patients who had high and low epididymal sperm 
      aneuploidy rate. CONCLUSIONS: Epididymal spermatozoa from obstructive azoospermic 
      patients had an elevated sex chromosome aneuploidy and diploidy rate. The 
      increased frequency of chromosomal abnormalities did not have a direct effect on 
      the ICSI outcome.
FAU - Sukcharoen, Nares
AU  - Sukcharoen N
AD  - Department of Obstetrics and Gynaecology, Faculty of Medicine, Chulalongkorn 
      University, Bangkok 10330, Thailand. sukcharoen@yahoo.com
FAU - Ngeamvijawat, Jiraporn
AU  - Ngeamvijawat J
FAU - Sithipravej, Tippawan
AU  - Sithipravej T
FAU - Promviengchai, Sakchai
AU  - Promviengchai S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
RN  - 0 (DNA Probes)
SB  - IM
MH  - *Aneuploidy
MH  - Chromosomes, Human, X/*genetics
MH  - Chromosomes, Human, Y/*genetics
MH  - DNA Probes
MH  - *Diploidy
MH  - Epididymis/*pathology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Oligospermia/etiology/*genetics
MH  - Ovulation Induction/methods
MH  - Pregnancy
MH  - Pregnancy Outcome
MH  - Retrospective Studies
MH  - Sperm Injections, Intracytoplasmic/methods
MH  - Spermatozoa/*cytology/pathology
PMC - PMC3455301
EDAT- 2003/06/19 05:00
MHDA- 2004/01/24 05:00
PMCR- 2004/05/01
CRDT- 2003/06/19 05:00
PHST- 2003/06/19 05:00 [pubmed]
PHST- 2004/01/24 05:00 [medline]
PHST- 2003/06/19 05:00 [entrez]
PHST- 2004/05/01 00:00 [pmc-release]
AID - 464273 [pii]
AID - 10.1023/a:1023674110940 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2003 May;20(5):196-203. doi: 10.1023/a:1023674110940.