PMID- 1281495
OWN - NLM
STAT- MEDLINE
DCOM- 19930115
LR  - 20211025
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 12
IP  - 12
DP  - 1992 Dec
TI  - Regulation of brain-derived neurotrophic factor and nerve growth factor mRNA in 
      primary cultures of hippocampal neurons and astrocytes.
PG  - 4793-9
AB  - Brain-derived neurotrophic factor (BDNF) and NGF are both expressed by neurons in 
      the hippocampus. In previous studies, it has been demonstrated that both BDNF and 
      NGF mRNA levels are regulated by neuronal activity. Upregulation is predominantly 
      regulated by the glutamate (NMDA and non-NMDA receptors); downregulation, 
      predominantly by the GABA system (Zafra et al., 1990, 1991). In neuronal cultures 
      of the rat hippocampus, potassium depolarization and kainic acid-mediated 
      increases in BDNF and NGF mRNA were eliminated in a dose-dependent manner by the 
      calcium channel blocker nifedipine. Conversely, calcium ionophores (Bay-K8644 and 
      ionomycin) augmented BDNF and NGF mRNA levels by a calmodulin-mediated mechanism. 
      In view of the fact that many potential modulators (conventional transmitters and 
      neuropeptides) of neuronal and astrocytic BDNF and NGF mRNA synthesis may act via 
      the adenylate cyclase system, we studied the effect of forskolin, an activator of 
      adenylate cyclase. Indeed, forskolin enhanced the effects of calcium ionophores 
      and kainic acid on BDNF and NGF mRNA levels. Cytokines, such as interleukin-1 and 
      transforming growth factor-beta 1, which have previously been shown to increase 
      NGF mRNA markedly in astrocytes, were without effect on neuronal BDNF and NGF 
      mRNA levels. In contrast to neuronal cultures, where the regulation of BDNF and 
      NGF mRNA was generally very similar, the regulation in astrocytes was distinctly 
      different. All the cytokines that produce a marked increase in NGF mRNA were 
      without effect on astrocyte BDNF mRNA levels, which under basic conditions were 
      below the detection limit. However, norepinephrine produced a marked elevation of 
      BDNF mRNA in astrocytes, an effect that was further enhanced by glutamate 
      receptor agonists.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Zafra, F
AU  - Zafra F
AD  - Department of Neurochemistry, Max Planck Institute for Psychiatry, 
      Planegg-Martinsried, Germany.
FAU - Lindholm, D
AU  - Lindholm D
FAU - Castren, E
AU  - Castren E
FAU - Hartikka, J
AU  - Hartikka J
FAU - Thoenen, H
AU  - Thoenen H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cytokines)
RN  - 0 (Glutamates)
RN  - 0 (Growth Substances)
RN  - 0 (Isoquinolines)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Piperazines)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 1F7A44V6OU (Colforsin)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 56092-81-0 (Ionomycin)
RN  - 71145-03-4 (3-Pyridinecarboxylic acid, 
      1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester)
RN  - 84477-87-2 (1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine)
RN  - 8OC22C1B99 (Quisqualic Acid)
RN  - E0399OZS9N (Cyclic AMP)
RN  - I9ZF7L6G2L (Nifedipine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - RWP5GA015D (Potassium)
RN  - SIV03811UC (Kainic Acid)
RN  - SY7Q814VUP (Calcium)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
MH  - 3-Pyridinecarboxylic acid, 
      1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl 
      ester/pharmacology
MH  - Animals
MH  - Astrocytes/drug effects/*metabolism
MH  - Brain-Derived Neurotrophic Factor
MH  - Calcium/metabolism/pharmacology
MH  - Cells, Cultured
MH  - Colforsin/pharmacology
MH  - Cyclic AMP/metabolism
MH  - Cytokines/*pharmacology
MH  - Glutamates/pharmacology
MH  - Glutamic Acid
MH  - Growth Substances/*pharmacology
MH  - Hippocampus/*metabolism
MH  - Ionomycin/pharmacology
MH  - Isoquinolines/pharmacology
MH  - Kainic Acid/pharmacology
MH  - Kinetics
MH  - Nerve Growth Factors/*biosynthesis/genetics
MH  - Nerve Tissue Proteins/*biosynthesis/genetics
MH  - Neurons/drug effects/*metabolism
MH  - Nifedipine/pharmacology
MH  - Norepinephrine/pharmacology
MH  - Piperazines/pharmacology
MH  - Potassium/pharmacology
MH  - Protein Kinase Inhibitors
MH  - Quisqualic Acid/pharmacology
MH  - RNA, Messenger/isolation & purification/*metabolism
MH  - Rats
MH  - Tetradecanoylphorbol Acetate/pharmacology
PMC - PMC6575775
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
PMCR- 1993/06/01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
PHST- 1993/06/01 00:00 [pmc-release]
AID - 10.1523/JNEUROSCI.12-12-04793.1992 [doi]
PST - ppublish
SO  - J Neurosci. 1992 Dec;12(12):4793-9. doi: 10.1523/JNEUROSCI.12-12-04793.1992.