PMID- 12815552
OWN - NLM
STAT- MEDLINE
DCOM- 20030922
LR  - 20190901
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 26
IP  - 3-4
DP  - 2003 Mar-Apr
TI  - Gene expression profiling in the HSV-1 latently infected mouse trigeminal ganglia 
      following hyperthermic stress.
PG  - 231-8
AB  - PURPOSE: To assess gene expression in herpes simplex virus type 1 (HSV-1) latent 
      mouse trigeminal ganglia (TG) at 6 and 24 hours after hyperthermic stress. 
      METHODS: Uninfected and HSV-1 latently infected mice were heat stressed (43 
      degrees C, 10 min). TG from six groups of mice were studied: 1) uninfected, not 
      stressed, 2) uninfected, heat-stressed, sacrificed at 6 hours after hyperthermia, 
      3) uninfected, heat-stressed, sacrificed at 24 hours after hyperthermia, 4) 
      latently infected, not stressed, 5) latently infected, heat-stressed, sacrificed 
      at 6 hours after hyperthermia, 6) latently infected, heat-stressed, sacrificed at 
      24 hours after hyperthermia. Poly A(+) mRNA from the TG of each group of mice was 
      reverse transcribed, labeled with (32)P, and incubated on a nylon gene array 
      membrane. The genes showing the largest signal-to-control changes (varying by a 
      factor of at least 1.27-fold) were considered to have undergone significant 
      change in expression. RESULTS: Six hours after heat stress the genes whose 
      expression was altered included the FK506-binding protein gene (decreased), the 
      T-complex protein 1 alpha subunit gene (increased), and the 94-kDa 
      glucose-regulated protein gene (increased in uninfected TG, decreased in infected 
      TG). Heat stress increased expression of the DNA excision repair protein ERCC5 
      gene 24 hours after the treatment. Genes previously reported to exhibit increased 
      transcription 1 hour after stress did not continue to show significant 
      transcriptional activation at 6 or 24 hours. CONCLUSION: Altered gene expression 
      at 6 and 24 hours after heat stress was different from previously reported 
      changes in gene expression 1 hour after hyperthermia in HSV-1 latently infected 
      mice.
FAU - Higaki, Shiro
AU  - Higaki S
AD  - Department of Ophthalmology (LSU Eye Center of Excellence), Louisiana State 
      University Health Sciences Center, New Orleans, USA.
FAU - Gebhardt, Bryan
AU  - Gebhardt B
FAU - Lukiw, Walter
AU  - Lukiw W
FAU - Thompson, Hilary
AU  - Thompson H
FAU - Hill, James
AU  - Hill J
LA  - eng
GR  - P30EY02377/EY/NEI NIH HHS/United States
GR  - R01AG18031/AG/NIA NIH HHS/United States
GR  - R01EY06311/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
SB  - IM
MH  - Animals
MH  - Female
MH  - Fever/*complications
MH  - *Gene Expression Profiling
MH  - Herpes Simplex/complications/*genetics
MH  - Herpesvirus 1, Human/genetics/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Oligonucleotide Array Sequence Analysis
MH  - Stress, Physiological/complications
MH  - Time Factors
MH  - Trigeminal Ganglion/metabolism/*virology
MH  - *Virus Latency
EDAT- 2003/06/20 05:00
MHDA- 2003/09/23 05:00
CRDT- 2003/06/20 05:00
PHST- 2003/06/20 05:00 [pubmed]
PHST- 2003/09/23 05:00 [medline]
PHST- 2003/06/20 05:00 [entrez]
AID - 10.1076/ceyr.26.3.231.14892 [doi]
PST - ppublish
SO  - Curr Eye Res. 2003 Mar-Apr;26(3-4):231-8. doi: 10.1076/ceyr.26.3.231.14892.