PMID- 1281763
OWN - NLM
STAT- MEDLINE
DCOM- 19930122
LR  - 20190706
IS  - 0009-8981 (Print)
IS  - 0009-8981 (Linking)
VI  - 211
IP  - 1-2
DP  - 1992 Oct 15
TI  - A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 3 
      (stromelysin-1) using monoclonal antibodies.
PG  - 59-72
AB  - A one-step sandwich enzyme immunoassay (EIA) for matrix metalloproteinase 3 
      (MMP-3; stromelysin-1) was developed. The assay system used two simultaneous 
      immunoreactions using a solid phase monoclonal antibody and a horseradish 
      peroxidase-labeled monoclonal antibody (Fab'). The sensitivity of the assay 
      system was 20 micrograms/l and linearity was obtained between 31 and 500 
      micrograms/l. The EIA system was capable of measuring both precursor and active 
      forms of MMP-3 as well as the forms of MMP-3 complexed with tissue inhibitors of 
      metalloproteinases. MMP-3 levels as measured by this assay are significantly 
      higher in the sera of patients with rheumatoid arthritis as compared to those of 
      healthy subjects and patients with osteoarthritis. Immunoblot analyses showed 
      that in the sera and synovial fluids of patients with rheumatoid arthritis, MMP-3 
      is present in the 59- and 57-kDa precursor forms.
FAU - Obata, K
AU  - Obata K
AD  - Department of Biotechnology, Fuji Chemical Industries, Ltd., Japan.
FAU - Iwata, K
AU  - Iwata K
FAU - Okada, Y
AU  - Okada Y
FAU - Kohrin, Y
AU  - Kohrin Y
FAU - Ohuchi, E
AU  - Ohuchi E
FAU - Yoshida, S
AU  - Yoshida S
FAU - Shinmei, M
AU  - Shinmei M
FAU - Hayakawa, T
AU  - Hayakawa T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Glycoproteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Protein Precursors)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 0 (alpha-Macroglobulins)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - *Antibodies, Monoclonal
MH  - Antibody Specificity
MH  - Cell Line
MH  - Female
MH  - Glycoproteins/metabolism
MH  - Humans
MH  - *Immunoenzyme Techniques/standards/statistics & numerical data
MH  - Matrix Metalloproteinase 3
MH  - Metalloendopeptidases/*analysis/blood/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neoplasm Proteins/metabolism
MH  - Protein Precursors/analysis/blood/immunology
MH  - Synovial Fluid/chemistry
MH  - Tissue Inhibitor of Metalloproteinase-2
MH  - Tissue Inhibitor of Metalloproteinases
MH  - alpha-Macroglobulins/metabolism
EDAT- 1992/10/15 00:00
MHDA- 1992/10/15 00:01
CRDT- 1992/10/15 00:00
PHST- 1992/10/15 00:00 [pubmed]
PHST- 1992/10/15 00:01 [medline]
PHST- 1992/10/15 00:00 [entrez]
AID - 0009-8981(92)90105-Y [pii]
AID - 10.1016/0009-8981(92)90105-y [doi]
PST - ppublish
SO  - Clin Chim Acta. 1992 Oct 15;211(1-2):59-72. doi: 10.1016/0009-8981(92)90105-y.