PMID- 12818190
OWN - NLM
STAT- MEDLINE
DCOM- 20030818
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1649
IP  - 1
DP  - 2003 Jun 26
TI  - Anticoagulant activity of M-LAO, L-amino acid oxidase purified from Agkistrodon 
      halys blomhoffii, through selective inhibition of factor IX.
PG  - 51-7
AB  - One of haemorrhagic toxins present in snake venoms is L-amino acid oxidase (LAO), 
      which catalyzes the oxidative deamination of L-amino acids with the generation of 
      hydrogen peroxide. Although it is widely accepted that LAO alters platelet 
      function, the effects of LAO on human blood coagulation remain largely unknown. 
      The present study demonstrated, for the first time, that M-LAO, LAO purified from 
      the venom of Agkistrodon halys blomhoffii (Japanese mamushi), possesses an 
      anticoagulant activity. Thrombelastography (TEG) showed that M-LAO significantly 
      delayed the onset and the progress of the coagulation process. In addition, the 
      enzyme prolonged the activated partial thromboplastin time (aPTT) 
      dose-dependently, but had little effect on the prothrombin time (PT), suggesting 
      that its principal activity was mediated in the intrinsic coagulation pathway. 
      Furthermore, M-LAO reduced factor IX procoagulant activity in a dose-dependent 
      manner and did not affect other coagulation factors. These results indicate that 
      M-LAO has an anticoagulant activity that impairs the intrinsic clotting by 
      inhibiting factor IX.
FAU - Sakurai, Yoshihiko
AU  - Sakurai Y
AD  - Department of Pediatrics, Nara Medical University, 840 Shijo-cho, Nara Kashihara 
      634-8522, Japan.
FAU - Shima, Midori
AU  - Shima M
FAU - Matsumoto, Tomoko
AU  - Matsumoto T
FAU - Takatsuka, Hideo
AU  - Takatsuka H
FAU - Nishiya, Katsumi
AU  - Nishiya K
FAU - Kasuda, Shogo
AU  - Kasuda S
FAU - Fujimura, Yoshihiro
AU  - Fujimura Y
FAU - Yoshioka, Akira
AU  - Yoshioka A
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Anticoagulants)
RN  - 0 (Crotalid Venoms)
RN  - 9001-28-9 (Factor IX)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.4.- (Amino Acid Oxidoreductases)
RN  - EC 1.4.3.2 (L-Amino Acid Oxidase)
SB  - IM
MH  - *Agkistrodon
MH  - Amino Acid Oxidoreductases/isolation & purification/*metabolism/*pharmacology
MH  - Animals
MH  - Anticoagulants/isolation & purification/metabolism/*pharmacology
MH  - Blood Coagulation Tests
MH  - Crotalid Venoms/enzymology
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Stability
MH  - Factor IX/*antagonists & inhibitors/metabolism
MH  - Humans
MH  - Hydrogen Peroxide/pharmacology
MH  - L-Amino Acid Oxidase
MH  - Prothrombin Time
MH  - Thrombelastography
EDAT- 2003/06/24 05:00
MHDA- 2003/08/19 05:00
CRDT- 2003/06/24 05:00
PHST- 2003/06/24 05:00 [pubmed]
PHST- 2003/08/19 05:00 [medline]
PHST- 2003/06/24 05:00 [entrez]
AID - S1570963903001572 [pii]
AID - 10.1016/s1570-9639(03)00157-2 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2003 Jun 26;1649(1):51-7. doi: 
      10.1016/s1570-9639(03)00157-2.