PMID- 12819063
OWN - NLM
STAT- MEDLINE
DCOM- 20030722
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 71
IP  - 7
DP  - 2003 Jul
TI  - Various types of Dirofilaria immitis polyproteins selectively induce a Th2-Type 
      immune response.
PG  - 3802-11
AB  - Dirofilaria immitis polyproteins (DiAgs) are found as 15-kDa monomeric and 30-kDa 
      dimeric forms in excretory-secretory products of the adult worm. We evaluated the 
      ability of various types of recombinant DiAg (rDiAg; V1 and V2 as monomers and 
      V1V2, V2V1, V1V1, and V2V2 as dimers) to influence Th1/Th2 immune responses. V1-, 
      V1Vx- and V2-, V2Vx-driven nonspecific immunoglobulin E (IgE) production peaked 
      at 21 and 14 days after administration, respectively. Dimer-induced IgE response 
      was an interesting biphasic pattern with the second peaks on days 35 (V2Vx) or 42 
      (V1Vx). Absolute amounts of nonspecific IgE production induced with monomers were 
      larger than those observed with dimers at the first peak. The magnitude of cell 
      expansion and interleukin-10 (IL-10) production in mesenteric lymph node (MLN) 
      B-cell induced with rDiAgs was linked to the levels of the first IgE peak in vivo 
      and IgE produced by rDiAg plus IL-4-stimulated B cells in vitro. All rDiAgs 
      failed to augment IgG2c production. V2 and V2Vx elicited IL-4 production by MLN 
      cells more rapidly than V1 and V1Vx. The inhibitory effect of rDiAg on gamma 
      interferon (IFN-gamma) production was stronger in monomers than in dimers. 
      Neutralization of IL-10 restored IFN-gamma production, whereas the expression of 
      IL-4 and IgE was partly prevented by depletion of IL-10. These results indicate 
      that monomer rather than dimer is an efficient form of DiAg and suggest that the 
      difference of IgE-inducing capacity among these DiAgs is closely associated with 
      the pattern of both B-cell activation and IL-4 production.
FAU - Tezuka, Hiroyuki
AU  - Tezuka H
AD  - Section of Environmental Parasitology, Department of Internation Health 
      Development, Division of Public Health, Graduate School, Tokyo Medical and Dental 
      University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
FAU - Imai, Shinjiro
AU  - Imai S
FAU - Hidano, Shinya
AU  - Hidano S
FAU - Tsukidate, Setsuko
AU  - Tsukidate S
FAU - Fujita, Koichiro
AU  - Fujita K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antigens, Helminth)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Polyproteins)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antigens, Helminth/*immunology
MH  - Dimerization
MH  - Dirofilaria immitis/*immunology
MH  - Female
MH  - Immunoglobulin E/biosynthesis
MH  - Immunoglobulin G/biosynthesis/classification
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-10/physiology
MH  - Interleukin-4/biosynthesis
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Polyproteins/*immunology
MH  - Th2 Cells/*immunology
PMC - PMC162011
EDAT- 2003/06/24 05:00
MHDA- 2003/07/23 05:00
PMCR- 2003/07/01
CRDT- 2003/06/24 05:00
PHST- 2003/06/24 05:00 [pubmed]
PHST- 2003/07/23 05:00 [medline]
PHST- 2003/06/24 05:00 [entrez]
PHST- 2003/07/01 00:00 [pmc-release]
AID - 1611 [pii]
AID - 10.1128/IAI.71.7.3802-3811.2003 [doi]
PST - ppublish
SO  - Infect Immun. 2003 Jul;71(7):3802-11. doi: 10.1128/IAI.71.7.3802-3811.2003.