PMID- 12820019 OWN - NLM STAT- MEDLINE DCOM- 20030909 LR - 20181113 IS - 0918-9440 (Print) IS - 0918-9440 (Linking) VI - 116 IP - 4 DP - 2003 Aug TI - Physical mapping of rDNA sequences in four karyotypes of Ranunculus silerifolius (Ranunculaceae). PG - 331-6 AB - The chromosomal locations of the 18S-5.8S-26S rDNA and 5S rDNA sequences were examined in four cytotypes of Ranunculus silerifolius (the Matsuyama, Mugi, Otaru, and Karatsu types) using fluorescence in situ hybridization (FISH). Using the 18S-5.8S-26S rDNA probe, one pair of probe hybridization sites was detected by FISH in the interstitial region corresponding to the secondary constriction on the short arm of a satellite chromosome (chromosome pair 6) in all four karyotypes. FISH using 5S rDNA identified one pair of sites. The 5S rDNA locus was on different chromosomes in the four karyotypes: in the interstitial region of the short arm of the largest metacentric chromosome (chromosome pair 1) in the Matsuyama type, in the interstitial region of the short arm of the subtelocentric chromosome (pair 2) in the Mugi and Otaru types, and in the interstitial region of the short arm of the metacentric chromosome (pair 2) in the Karatsu type. This physical mapping of the 5S rDNA provides valuable information about karyotype evolution in R. silerifolius. Possible mechanisms of chromosome evolution are discussed. FAU - Takahashi, Chigusa AU - Takahashi C AD - Faculty of Education and Regional Sciences, Tottori University, Tottori 680-8551, Japan. chigusa@fed.tottori-u.ac.jp LA - eng PT - Journal Article DEP - 20030619 PL - Japan TA - J Plant Res JT - Journal of plant research JID - 9887853 RN - 0 (DNA, Plant) RN - 0 (DNA, Ribosomal) SB - IM MH - *DNA, Plant MH - *DNA, Ribosomal MH - In Situ Hybridization, Fluorescence MH - Karyotyping MH - Physical Chromosome Mapping MH - Ranunculus/*genetics EDAT- 2003/06/24 05:00 MHDA- 2003/09/10 05:00 CRDT- 2003/06/24 05:00 PHST- 2003/02/17 00:00 [received] PHST- 2003/04/18 00:00 [accepted] PHST- 2003/06/24 05:00 [pubmed] PHST- 2003/09/10 05:00 [medline] PHST- 2003/06/24 05:00 [entrez] AID - 10.1007/s10265-003-0104-8 [doi] PST - ppublish SO - J Plant Res. 2003 Aug;116(4):331-6. doi: 10.1007/s10265-003-0104-8. Epub 2003 Jun 19.