PMID- 12821046
OWN - NLM
STAT- MEDLINE
DCOM- 20040311
LR  - 20190817
IS  - 0168-8278 (Print)
IS  - 0168-8278 (Linking)
VI  - 39
IP  - 1
DP  - 2003 Jul
TI  - Chronic alcohol exposure sensitizes mice to galactosamine-induced liver injury 
      through enhanced keratinocyte chemoattractant and defective IL-10 production.
PG  - 68-76
AB  - BACKGROUND/AIMS: Alcohol sensitizes the liver to several injuries. The mechanisms 
      leading to this sensitization are poorly defined. In the present study, we 
      developed a mouse model of chronic exposure to alcohol vapours that sensitize 
      mice to galactosamine (GAL) liver injury. METHODS: C57BL/6 mice were exposed to 
      ethanol vapours for 10 days. Liver injury was induced by intraperitoneal 
      injection of GAL (1 g/kg) and mice were killed 24 h later. RESULTS: GAL challenge 
      after ethanol pre-treatment significantly raised serum alanine aminotransaminase 
      (ALT) levels and enhanced liver inflammation when compared with the controls (GAL 
      alone). Serum keratinocyte chemoattractant (KC) and monocyte chemoattractant 
      protein-1 (MCP-1) levels were significantly increased in the GAL+ethanol group. 
      On the contrary, serum interleukin 10 (IL-10) levels were lower than in controls. 
      Anti-KC, anti-tumour necrosis factor alpha antibodies and intestinal 
      decontamination significantly protected mice from liver injury. In 
      GAL+ethanol-treated mice, IL-10 treatment reduced ALT release, KC and MCP-1 serum 
      and hepatic mRNA levels, and improved liver inflammation. CONCLUSIONS: 
      Enhancement of GAL-induced liver injury by ethanol is associated with an 
      imbalance between proinflammatory cytokines and the anti-inflammatory cytokine 
      IL-10 and depends on gut bacterial flora.
FAU - Sermon, Filip
AU  - Sermon F
AD  - Laboratory of Experimental Gastroenterology, Hopital Erasme, Universite Libre de 
      Bruxelles, 808 Route de Lennik, 1070 Brussels, Belgium.
FAU - Le Moine, Olivier
AU  - Le Moine O
FAU - Gustot, Thierry
AU  - Gustot T
FAU - Quertinmont, Eric
AU  - Quertinmont E
FAU - Louis, Hubert
AU  - Louis H
FAU - Nagy, Nathalie
AU  - Nagy N
FAU - Degraef, Chantal
AU  - Degraef C
FAU - Deviere, Jacques
AU  - Deviere J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Hepatol
JT  - Journal of hepatology
JID - 8503886
RN  - 0 (Central Nervous System Depressants)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 3K9958V90M (Ethanol)
RN  - 7535-00-4 (Galactosamine)
SB  - IM
MH  - Administration, Inhalation
MH  - Animals
MH  - Central Nervous System Depressants/pharmacology
MH  - Chemokine CCL2/*metabolism
MH  - Chronic Disease
MH  - Drug Interactions
MH  - Ethanol/pharmacology
MH  - Female
MH  - Galactosamine/*pharmacology
MH  - Interleukin-10/*metabolism/pharmacology
MH  - Intestines/microbiology
MH  - Keratinocytes/cytology
MH  - Liver Diseases, Alcoholic/*immunology/*metabolism/pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2003/06/25 05:00
MHDA- 2004/03/12 05:00
CRDT- 2003/06/25 05:00
PHST- 2003/06/25 05:00 [pubmed]
PHST- 2004/03/12 05:00 [medline]
PHST- 2003/06/25 05:00 [entrez]
AID - S0168827803001867 [pii]
AID - 10.1016/s0168-8278(03)00186-7 [doi]
PST - ppublish
SO  - J Hepatol. 2003 Jul;39(1):68-76. doi: 10.1016/s0168-8278(03)00186-7.