PMID- 12821652
OWN - NLM
STAT- MEDLINE
DCOM- 20031117
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 278
IP  - 39
DP  - 2003 Sep 26
TI  - The orphan nuclear receptor Rev-Erbalpha is a peroxisome proliferator-activated 
      receptor (PPAR) gamma target gene and promotes PPARgamma-induced adipocyte 
      differentiation.
PG  - 37672-80
AB  - Rev-Erbalpha (NR1D1) is an orphan nuclear receptor encoded on the opposite strand 
      of the thyroid receptor alpha gene. Rev-Erbalpha mRNA is induced during adipocyte 
      differentiation of 3T3-L1 cells, and its expression is abundant in rat adipose 
      tissue. Peroxisome proliferator-activated receptor gamma (PPARgamma) (NR1C3) is a 
      nuclear receptor controlling adipocyte differentiation and insulin sensitivity. 
      Here we show that Rev-Erbalpha expression is induced by PPARgamma activation with 
      rosiglitazone in rat epididymal and perirenal adipose tissues in vivo as well as 
      in 3T3-L1 adipocytes in vitro. Furthermore, activated PPARgamma induces 
      Rev-Erbalpha promoter activity by binding to the direct repeat (DR)-2 response 
      element Rev-DR2. Mutations of the 5' or 3' half-sites of the response element 
      totally abrogated PPARgamma binding and transcriptional activation, identifying 
      this site as a novel type of functional PPARgamma response element. Finally, 
      ectopic expression of Rev-Erbalpha in 3T3-L1 preadipocytes potentiated adipocyte 
      differentiation induced by the PPARgamma ligand rosiglitazone. These results 
      identify Rev-Erbalpha as a target gene of PPARgamma in adipose tissue and 
      demonstrate a role for this nuclear receptor as a promoter of adipocyte 
      differentiation.
FAU - Fontaine, Coralie
AU  - Fontaine C
AD  - UR545 INSERM, Departement d'Atherosclerose, 1, rue Calmette, Institut Pasteur de 
      Lille, 59019 Lille, France.
FAU - Dubois, Guillaume
AU  - Dubois G
FAU - Duguay, Yannick
AU  - Duguay Y
FAU - Helledie, Torben
AU  - Helledie T
FAU - Vu-Dac, Ngoc
AU  - Vu-Dac N
FAU - Gervois, Philippe
AU  - Gervois P
FAU - Soncin, Fabrice
AU  - Soncin F
FAU - Mandrup, Susanne
AU  - Mandrup S
FAU - Fruchart, Jean-Charles
AU  - Fruchart JC
FAU - Fruchart-Najib, Jamila
AU  - Fruchart-Najib J
FAU - Staels, Bart
AU  - Staels B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030623
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CCAAT-Enhancer-Binding Protein-alpha)
RN  - 0 (CCAAT-Enhancer-Binding Protein-beta)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (NR1D1 protein, human)
RN  - 0 (Nr1d1 protein, rat)
RN  - 0 (Nuclear Receptor Subfamily 1, Group D, Member 1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Adipocytes/*cytology
MH  - Animals
MH  - Binding Sites
MH  - CCAAT-Enhancer-Binding Protein-alpha/physiology
MH  - CCAAT-Enhancer-Binding Protein-beta/physiology
MH  - Cell Differentiation
MH  - DNA-Binding Proteins/*genetics
MH  - Dimerization
MH  - Humans
MH  - Male
MH  - Nuclear Receptor Subfamily 1, Group D, Member 1
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Cytoplasmic and Nuclear/*genetics/*physiology
MH  - Receptors, Retinoic Acid/metabolism
MH  - Retinoid X Receptors
MH  - Transcription Factors/metabolism/*physiology
MH  - Transcription, Genetic
EDAT- 2003/06/25 05:00
MHDA- 2003/12/03 05:00
CRDT- 2003/06/25 05:00
PHST- 2003/06/25 05:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/06/25 05:00 [entrez]
AID - S0021-9258(20)83331-6 [pii]
AID - 10.1074/jbc.M304664200 [doi]
PST - ppublish
SO  - J Biol Chem. 2003 Sep 26;278(39):37672-80. doi: 10.1074/jbc.M304664200. Epub 2003 
      Jun 23.