PMID- 1282379
OWN - NLM
STAT- MEDLINE
DCOM- 19930208
LR  - 20131121
IS  - 0925-5710 (Print)
IS  - 0925-5710 (Linking)
VI  - 56
IP  - 3
DP  - 1992 Dec
TI  - Analysis of leukemic cell activity with a simple acridine orange staining method.
PG  - 197-204
AB  - Leukemic cells from 28 patients with acute lymphoblastic leukemia (ALL) were 
      supravitally stained with acridine orange (AO). Based on the staining 
      characteristics of their cells, the patients were divided into an orange 
      cell-dominant group (15 patients) and a green cell-dominant group (13 patients). 
      The prognosis was better in the former than in the latter group. Orange cells 
      which had high RNA contents were observed, while the proportion of dividing cells 
      was high. Supravital staining with AO is a very simple and useful method for 
      assessing the metabolic activity of leukemic cells in ALL; this method may thus 
      be useful in assessing the prognosis of these patients.
FAU - Urashima, M
AU  - Urashima M
AD  - Department of Pediatrics, Tokyo Jikei University School of Medicine, Japan.
FAU - Hoshi, Y
AU  - Hoshi Y
FAU - Akatsuka, J
AU  - Akatsuka J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
RN  - 0 (DNA, Neoplasm)
RN  - 0 (RNA, Neoplasm)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - DNA, Neoplasm/*analysis
MH  - Female
MH  - Fluorescence
MH  - Follow-Up Studies
MH  - Humans
MH  - Infant
MH  - Leukemia, Promyelocytic, Acute/pathology
MH  - Lysosomes/ultrastructure
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Neoplastic Stem Cells/*chemistry/ultrastructure
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics/mortality/*pathology
MH  - Prognosis
MH  - RNA, Neoplasm/*analysis
MH  - *Staining and Labeling
MH  - Survival Analysis
MH  - Tumor Cells, Cultured
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
PST - ppublish
SO  - Int J Hematol. 1992 Dec;56(3):197-204.