PMID- 12824008
OWN - NLM
STAT- MEDLINE
DCOM- 20030711
LR  - 20191107
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 21
IP  - 5
DP  - 2003 Mar 7
TI  - Immunocytochemical localization of cytokines and inducible nitric oxide synthase 
      (iNOS) in oral mucosa and lymph nodes of patients with paracoccidioidomycosis.
PG  - 234-41
AB  - Paracoccidioidomycosis (PCM) is a deep mycosis caused by Paracoccidioides 
      brasiliensis, with high incidence in Brazil. In order to examine the immune 
      response in lesional tissue from patients with PCM, we analyzed cytokines as well 
      as the phenotype of the cell infiltrate. Paraffin-embedded tissue from the oral 
      mucosa of eight patients with the localized adult form (AF) of PCM and from the 
      lymph nodes of 10 patients with the juvenile form (JF) of PCM was analyzed by 
      immunohistochemistry to detect tumor necrosis factor-alpha (TNF-alpha), inducible 
      nitric oxide synthase (iNOS), transforming growth factor-beta (TGF-beta) and 
      interleukin-10 (IL-10). Most of the inflammatory cells in the lymph nodes were 
      CD68+ (macrophages, epithelioid and giant cells), while a mixed infiltrate with 
      macrophages, plasma cells and neutrophils was detected in the oral mucosa. 
      TNF-alpha as well as iNOS expression was similar in lymph nodes and oral mucosa, 
      whereas TGF-beta and IL-10 were observed in a larger number of macrophages, 
      epithelioid and giant cells in the lymph nodes, where numerous yeast cells were 
      visualized. The higher expression of anti-inflammatory cytokines (IL-10 and 
      TGF-beta) in lesions of patients with the JF of PCM (lymph nodes) may represent a 
      mechanism by which the fungus evades the host immune response, contributing to a 
      more severe and disseminated form of the disease.
FAU - Neworal, E P M
AU  - Neworal EP
AD  - Department of Clinical Pathology, State University of Campinas Medical School, 
      Campinas, SP, Brazil.
FAU - Altemani, A
AU  - Altemani A
FAU - Mamoni, R L
AU  - Mamoni RL
FAU - Noronha, I L
AU  - Noronha IL
FAU - Blotta, M H S L
AU  - Blotta MH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Cytokines)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - EC 1.14.13.39 (NOS2 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cytokines/*analysis/immunology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-10/analysis/immunology
MH  - Lymph Nodes/*chemistry/*enzymology
MH  - Macrophages/chemistry
MH  - Male
MH  - Middle Aged
MH  - Mouth Mucosa/*chemistry/*enzymology
MH  - Nitric Oxide Synthase/immunology/*metabolism
MH  - Nitric Oxide Synthase Type II
MH  - Paracoccidioides
MH  - Paracoccidioidomycosis/enzymology/*metabolism
MH  - Transforming Growth Factor beta/analysis/immunology
MH  - Tumor Necrosis Factor-alpha/analysis/immunology
EDAT- 2003/06/26 05:00
MHDA- 2003/07/12 05:00
CRDT- 2003/06/26 05:00
PHST- 2003/06/26 05:00 [pubmed]
PHST- 2003/07/12 05:00 [medline]
PHST- 2003/06/26 05:00 [entrez]
AID - S1043466603000516 [pii]
AID - 10.1016/s1043-4666(03)00051-6 [doi]
PST - ppublish
SO  - Cytokine. 2003 Mar 7;21(5):234-41. doi: 10.1016/s1043-4666(03)00051-6.