PMID- 12824057
OWN - NLM
STAT- MEDLINE
DCOM- 20030905
LR  - 20190827
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 115
IP  - 1
DP  - 2003 Jul 4
TI  - Temporal changes in the expression of brain-derived neurotrophic factor mRNA in 
      the ventromedial nucleus of the hypothalamus of the developing rat brain.
PG  - 69-77
AB  - Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family, 
      which is important for the growth, differentiation, and survival of neurons 
      during development. We have performed a detailed mapping of BDNF mRNA in the 
      neonatal rat brain using a quantitative in situ hybridization technique. At 
      postnatal day (PND) 4, hypothalamic structures showed only modest expression of 
      BDNF mRNA, with the exception of the ventromedial nucleus (VMN), where expression 
      was higher than that detected in the hippocampus. Abundant BDNF mRNA was also 
      found in the bed nucleus of the anterior commissure, retrosplenial granular 
      cortex, and the posteroventral part of the medial amygdaloid nucleus. Messenger 
      RNAs encoding other neurotrophins, including nerve growth factor (NGF) and 
      neurotrophin-3 (NT-3) and the BDNF receptor trkB, were not selectively localized 
      in neonatal VMN. During subsequent developmental stages, BDNF mRNA expression in 
      the VMN changed dynamically, peaking at PND 4 and falling to minimal levels in 
      the adult brain. In contrast, the low levels of BDNF mRNA observed in the CA3 
      region of the hippocampus increased to adult levels following PND 10. As the VMN 
      undergoes sexual differentiation, we compared BDNF, NGF, NT-3, and trkB mRNA 
      expression in the VMN in males and females at embryonic day 20 and PND 4, but 
      found no differences between them. These results suggest that localized and high 
      level expression of BDNF mRNA in the neonatal VMN plays an important role in its 
      neural organization and functional development.
FAU - Sugiyama, Nobuhiro
AU  - Sugiyama N
AD  - Department of Neuropsychiatry, University of Yamanashi Faculty of Medicine, 
      Tamaho, Yamanashi 409-3898, Japan.
FAU - Kanba, Shigenobu
AU  - Kanba S
FAU - Arita, Jun
AU  - Arita J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Aging/*genetics
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Cell Differentiation/*genetics
MH  - Female
MH  - Fetus
MH  - Gene Expression Regulation, Developmental/*genetics
MH  - Male
MH  - Nerve Growth Factor/genetics
MH  - Neurons/cytology/*metabolism
MH  - Neurotrophin 3/genetics
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkB/genetics
MH  - Sex Characteristics
MH  - Sex Differentiation/genetics
MH  - Time Factors
MH  - Up-Regulation/genetics
MH  - Ventromedial Hypothalamic Nucleus/embryology/growth & development/*metabolism
EDAT- 2003/06/26 05:00
MHDA- 2003/09/06 05:00
CRDT- 2003/06/26 05:00
PHST- 2003/06/26 05:00 [pubmed]
PHST- 2003/09/06 05:00 [medline]
PHST- 2003/06/26 05:00 [entrez]
AID - S0169328X03001840 [pii]
AID - 10.1016/s0169-328x(03)00184-0 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 2003 Jul 4;115(1):69-77. doi: 
      10.1016/s0169-328x(03)00184-0.