PMID- 12825178
OWN - NLM
STAT- MEDLINE
DCOM- 20030813
LR  - 20061115
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 188
IP  - 1
DP  - 2003 Jul 1
TI  - Polymorphic membrane protein (PMP) 20 and PMP 21 of Chlamydia pneumoniae induce 
      proinflammatory mediators in human endothelial cells in vitro by activation of 
      the nuclear factor-kappaB pathway.
PG  - 108-13
AB  - We tested whether polymorphic membrane proteins (PMPs) of Chlamydia pneumoniae 
      might play a role in triggering an inflammatory response in human endothelial 
      cells. Of 15 purified, recombinant chlamydial PMPs tested, 2 (PMP 20 and PMP 21) 
      dose-dependently increased the production of the inflammatory mediators 
      interleukin (IL)-6 and monocyte chemoattractant protein-1 (MCP-1), in cultured 
      human endothelial cells; production of IL-8 was also increased. When endothelial 
      cells were infected by live C. pneumoniae, an increase in the production of IL-6, 
      IL-8, and MCP-1 was seen. We used adenovirus-induced overexpression of 
      IkappaBalpha-an inhibitor of nuclear factor (NF)-kappaB-to demonstrate that PMP 
      20 and PMP 21 increase the production of IL-6 and MCP-1 in human endothelial 
      cells by activation of the NF-kappaB pathway, because, in cells overexpressing 
      IkappaBalpha, treatment with the respective PMP did not result in increased 
      production of IL-6 and MCP-1. Thus, C. pneumoniae could, by interactions of its 
      PMPs with the endothelium, contribute to the process of vascular injury during 
      the development and progression of atherosclerotic lesions.
FAU - Niessner, Alexander
AU  - Niessner A
AD  - Department of Internal Medicine II, University of Vienna, Vienna, Austria.
FAU - Kaun, Christoph
AU  - Kaun C
FAU - Zorn, Gerlinde
AU  - Zorn G
FAU - Speidl, Walter
AU  - Speidl W
FAU - Turel, Zeynep
AU  - Turel Z
FAU - Christiansen, Gunna
AU  - Christiansen G
FAU - Pedersen, Anna-Sofie
AU  - Pedersen AS
FAU - Birkelund, Svend
AU  - Birkelund S
FAU - Simon, Susan
AU  - Simon S
FAU - Georgopoulos, Apostolos
AU  - Georgopoulos A
FAU - Graninger, Wolfgang
AU  - Graninger W
FAU - de Martin, Rainer
AU  - de Martin R
FAU - Lipp, Joachim
AU  - Lipp J
FAU - Binder, Bernd R
AU  - Binder BR
FAU - Maurer, Gerald
AU  - Maurer G
FAU - Huber, Kurt
AU  - Huber K
FAU - Wojta, Johann
AU  - Wojta J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030612
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Bacterial Outer Membrane Proteins/*pharmacology
MH  - Cells, Cultured
MH  - Chemokine CCL2/biosynthesis
MH  - Chlamydophila pneumoniae/*chemistry
MH  - Cytokines/*biosynthesis
MH  - Endothelium, Vascular/*drug effects/*metabolism
MH  - Humans
MH  - Inflammation/metabolism
MH  - Interleukin-6/biosynthesis
MH  - Interleukin-8/biosynthesis
MH  - NF-kappa B/*metabolism
MH  - Signal Transduction/drug effects
MH  - Umbilical Veins/cytology
EDAT- 2003/06/26 05:00
MHDA- 2003/08/14 05:00
CRDT- 2003/06/26 05:00
PHST- 2002/10/22 00:00 [received]
PHST- 2003/02/12 00:00 [accepted]
PHST- 2003/06/26 05:00 [pubmed]
PHST- 2003/08/14 05:00 [medline]
PHST- 2003/06/26 05:00 [entrez]
AID - JID30236 [pii]
AID - 10.1086/375827 [doi]
PST - ppublish
SO  - J Infect Dis. 2003 Jul 1;188(1):108-13. doi: 10.1086/375827. Epub 2003 Jun 12.