PMID- 12826583
OWN - NLM
STAT- MEDLINE
DCOM- 20031211
LR  - 20190606
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 69
IP  - 4
DP  - 2003 Oct
TI  - Dendritic cells in the human decidua.
PG  - 1438-46
AB  - Dendritic cells (DCs) in the pregnant human uterine mucosa have been poorly 
      characterized, although they are likely to regulate immune responses to both 
      placental trophoblast cells and uterine infections. In this study an HLA-DR+, 
      CD11c+ lin- (CD3-, CD19-, CD56-, CD14-) population has been identified by 
      three-color flow cytometry. The cell isolates were prepared either by collagenase 
      digestion or mechanically from first-trimester decidual tissue. The decidual DCs 
      comprised approximately 1.7% of CD45+ cells in the isolates and had the phenotype 
      of immature myeloid DCs. No CD1a+ Langerhans cells or CD123+ plasmacytoid DCs 
      were detected. The decidual DCs were DC-SIGN-, DEC-205+, CD40+. Two subsets could 
      be distinguished on the basis of relative expression of HLA-DR, which also 
      differed in expression of DC-activation markers. The DCs were identified in situ 
      by immunohistology by DEC-205 staining. Cells with dendritic processes were found 
      scattered through both the decidua basalis (in which trophoblast cells are 
      infiltrating) and the decidua parietalis. They were also visible in 
      endothelial-lined spaces. This is the first study to identify and describe the 
      phenotype and distribution of human decidual DCs.
FAU - Gardner, Lucy
AU  - Gardner L
AD  - Research Group in Human Reproductive Immunobiology, Department of Pathology, 
      University of Cambridge, Cambridge CB2 1QP, United Kingdom.
FAU - Moffett, Ashley
AU  - Moffett A
LA  - eng
GR  - R01 39670/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030625
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Antigens, CD19)
RN  - 0 (CD11c Antigen)
RN  - 0 (CD3 Complex)
RN  - 0 (CD56 Antigen)
RN  - 0 (Carrier Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (lin protein, Drosophila)
SB  - IM
MH  - Antigens, CD19/metabolism
MH  - CD11c Antigen/metabolism
MH  - CD3 Complex/metabolism
MH  - CD56 Antigen/metabolism
MH  - Carrier Proteins/metabolism
MH  - Decidua/*cytology/physiology
MH  - Dendritic Cells/cytology/*physiology
MH  - *Drosophila Proteins
MH  - Female
MH  - HLA-DR Antigens/metabolism
MH  - Humans
MH  - Lipopolysaccharide Receptors/metabolism
MH  - Macrophages/cytology/physiology
MH  - Mucous Membrane/cytology
MH  - Phenotype
MH  - Pregnancy
MH  - Pregnancy Trimester, First
EDAT- 2003/06/27 05:00
MHDA- 2003/12/12 05:00
CRDT- 2003/06/27 05:00
PHST- 2003/06/27 05:00 [pubmed]
PHST- 2003/12/12 05:00 [medline]
PHST- 2003/06/27 05:00 [entrez]
AID - biolreprod.103.017574 [pii]
AID - 10.1095/biolreprod.103.017574 [doi]
PST - ppublish
SO  - Biol Reprod. 2003 Oct;69(4):1438-46. doi: 10.1095/biolreprod.103.017574. Epub 
      2003 Jun 25.