PMID- 1282721
OWN - NLM
STAT- MEDLINE
DCOM- 19930211
LR  - 20191028
IS  - 1011-6524 (Print)
IS  - 1011-6524 (Linking)
VI  - 15
IP  - 6
DP  - 1992 Nov-Dec
TI  - Effect of bradykinin on the cytosolic free calcium activity and phosphoinositol 
      turnover in human glomerular epithelial cells.
PG  - 277-88
AB  - The effect of bradykinin (BK) on the intracellular free calcium activity [Ca2+]i 
      and phosphoinositide (PI) turnover was investigated in human glomerular 
      epithelial cells (GEC) in culture. Human GEC exhibited a baseline [Ca2+]i of 114 
      +/- 3 nmol (n = 81). BK (ED50 10(-9) mol/l) caused a rapid and transient increase 
      in [Ca2+]i, which could also be observed in the absence of extracellular calcium. 
      The effect of BK (10(-8) mol/l) on the [Ca2+]i was inhibited by the BK2 
      antagonist Hoe 140 (IC50 10(-8) mol/l). BK also induced PI turnover in a time- 
      and dose-dependent manner. A transient increase in (1,4,5)-inositol-triphosphate 
      (InsP3) formation from 1,445 +/- 119 to 4,629 +/- 323 cpm occurred after 5 s. 
      Stimulation of protein kinase C (PKC) by short-term preincubation (15 min) of 
      human GEC with phorbol-12-myristate-13-acetate (PMA) induced a dose-dependent 
      inhibition of the BK-stimulated (10(-7) mol/l) inositol-phosphate formation. 
      Downregulation of PKC by preincubation of human GEC with PMA (24 h, 10(-6) mol/l) 
      or inhibition of PKC by pretreatment with staurosporin (1 h, 10(-6) mol/l) 
      resulted in a slight but significant augmentation of the BK-induced InsP3 
      stimulation. The data indicate that BK induces stimulation of [Ca2+]i and PI 
      turnover via a BK2 receptor in human GEC. PKC might exert a negative feedback 
      function for the BK-induced PI turnover.
FAU - Pavenstadt, H
AU  - Pavenstadt H
AD  - Department of Medicine, University of Freiburg, FRG.
FAU - Spath, M
AU  - Spath M
FAU - Fiedler, C
AU  - Fiedler C
FAU - Fischer, R
AU  - Fischer R
FAU - Schlunck, G
AU  - Schlunck G
FAU - Wanner, C
AU  - Wanner C
FAU - Schollmeyer, P
AU  - Schollmeyer P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Ren Physiol Biochem
JT  - Renal physiology and biochemistry
JID - 8906670
RN  - 0 (Phosphatidylinositols)
RN  - 7PG89G35Q7 (icatibant)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - S8TIM42R2W (Bradykinin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Bradykinin/analogs & derivatives/antagonists & inhibitors/*pharmacology
MH  - Calcium/*metabolism
MH  - Cells, Cultured
MH  - Cytosol/drug effects/*metabolism
MH  - Epithelial Cells
MH  - Epithelium/drug effects/metabolism
MH  - Feedback
MH  - Humans
MH  - Kidney Glomerulus/cytology/*drug effects/metabolism
MH  - Phosphatidylinositols/*metabolism
MH  - Protein Kinase C/metabolism
MH  - Signal Transduction
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
AID - 10.1159/000173464 [doi]
PST - ppublish
SO  - Ren Physiol Biochem. 1992 Nov-Dec;15(6):277-88. doi: 10.1159/000173464.