PMID- 12827381
OWN - NLM
STAT- MEDLINE
DCOM- 20040319
LR  - 20181113
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 112
IP  - 1
DP  - 2003 Jul
TI  - W-derived BAC probes as a new tool for identification of the W chromosome and its 
      aberrations in Bombyx mori.
PG  - 48-55
AB  - We isolated four W chromosome-derived bacterial artificial chromosome (W-BAC) 
      clones from Bombyx mori BAC libraries by the polymerase chain reaction and used 
      them as probes for fluorescence in situ hybridization (FISH) on chromosome 
      preparations from B. mori females. All four W-BAC probes surprisingly highlighted 
      the whole wild-type W sex chromosome and also identified the entire original 
      W-chromosomal region in W chromosome-autosome translocation mutants. This is the 
      first successful identification of a single chromosome by means of BAC-FISH in 
      species with holokinetic chromosomes. Genomic in situ hybridization (GISH) by 
      using female-derived genomic probes highlighted the W chromosome in a similar 
      chromosome-painting manner. Besides the W, hybridization signals of W-BAC probes 
      also occurred in telomeric and/or subtelomeric regions of the autosomes. These 
      signals coincided well with those of female genomic probes except one additional 
      GISH signal that was observed in a large heterochromatin block of one autosome 
      pair. Our results support the opinion that the B. mori W chromosome accumulated 
      transposable elements and other repetitive sequences that also occur, but 
      scattered, elsewhere in the respective genome.
FAU - Sahara, Ken
AU  - Sahara K
AD  - Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido 
      University N9, W9, Kita-ku, Sapporo 060-8589, Japan. sahara@abs.agr.hokudai.ac.jp
FAU - Yoshido, Atsuo
AU  - Yoshido A
FAU - Kawamura, Naoko
AU  - Kawamura N
FAU - Ohnuma, Akio
AU  - Ohnuma A
FAU - Abe, Hiroaki
AU  - Abe H
FAU - Mita, Kazuei
AU  - Mita K
FAU - Oshiki, Toshikazu
AU  - Oshiki T
FAU - Shimada, Toru
AU  - Shimada T
FAU - Asano, Shin-Ichiro
AU  - Asano S
FAU - Bando, Hisanori
AU  - Bando H
FAU - Yasukochi, Yuji
AU  - Yasukochi Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030624
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (DNA Probes)
SB  - IM
MH  - Animals
MH  - Bombyx/*genetics
MH  - Chromosomes, Artificial, Bacterial/*genetics
MH  - *DNA Probes
MH  - Female
MH  - Gene Library
MH  - Genome
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Karyotyping
MH  - Male
MH  - *Sex Chromosome Aberrations
MH  - *Sex Chromosomes
EDAT- 2003/06/27 05:00
MHDA- 2004/03/20 05:00
CRDT- 2003/06/27 05:00
PHST- 2003/02/27 00:00 [received]
PHST- 2003/05/22 00:00 [revised]
PHST- 2003/05/28 00:00 [accepted]
PHST- 2003/06/27 05:00 [pubmed]
PHST- 2004/03/20 05:00 [medline]
PHST- 2003/06/27 05:00 [entrez]
AID - 10.1007/s00412-003-0245-5 [doi]
PST - ppublish
SO  - Chromosoma. 2003 Jul;112(1):48-55. doi: 10.1007/s00412-003-0245-5. Epub 2003 Jun 
      24.