PMID- 12827467
OWN - NLM
STAT- MEDLINE
DCOM- 20030923
LR  - 20220317
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 148
IP  - 7
DP  - 2003 Jul
TI  - Pathotypical and genotypical characterization of strains of Newcastle disease 
      virus isolated from outbreaks in chicken and goose flocks in some regions of 
      China during 1985-2001.
PG  - 1387-403
AB  - Twenty-nine strains of Newcastle disease virus (NDV) isolated from outbreaks in 
      chicken and goose flocks in several regions of China during 1985-2001 were 
      characterized pathotypically and genotypically. All except one of these strains 
      were velogenic. For genotyping, phylogenetic tree analysis (nt 47-420), 
      restriction site mapping (nt 334-1682) and residue substitution analysis 
      (residues 4-124) of the F gene were performed using sequences of our isolates and 
      sequences of selected NDV strains from GenBank. The results revealed that most of 
      these newly characterized strains belonged to six novel genetic groups that were 
      designated as VIf, VIg, VIIc, VIId, VIIe and IX. The genotype IX viruses, to 
      which the China challenge strain F48E8 used for vaccine evaluation belonged, were 
      found only in China and still induced sporadic infections in certain areas. 
      Isolates belonging to group VIf and VIg were distinct from previously reported 
      members of genotype VI, with genetic distance from 2.5 to 12.1%. Subgenotype 
      VIIc, VIId and VIIe viruses, which were distributed in clusters in the 
      phylogenetic tree distinct from members of subgenotypes VIIa and VIIb, were 
      responsible for disease outbreaks in chicken and goose flocks and circulated 
      predominantly in southern China in recent years. Finally, cross-protective 
      testing showed that specific-pathogen free (SPF) chickens vaccinated with La Sota 
      vaccines can be fully protected against challenge by strains from genetic groups 
      VIb, VIg, VIId and IX, indicating that the antigenic differences between strains 
      of various genotypes are insufficient to change the cross-protection conferred by 
      the commonly used vaccine.
FAU - Liu, X F
AU  - Liu XF
AD  - Animal Infectious Disease Laboratory, School of Veterinary Medicine, Yangzhou 
      University, Yangzhou, Jiangsu, China. xfliu@mail.yzu.edu.cn
FAU - Wan, H Q
AU  - Wan HQ
FAU - Ni, X X
AU  - Ni XX
FAU - Wu, Y T
AU  - Wu YT
FAU - Liu, W B
AU  - Liu WB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chickens
MH  - China/epidemiology
MH  - DNA Primers
MH  - Disease Outbreaks/*veterinary
MH  - Geese
MH  - Genotype
MH  - Newcastle Disease/*epidemiology/virology
MH  - Newcastle disease virus/genetics/isolation & purification/*pathogenicity
MH  - Poultry Diseases/genetics/*virology
MH  - RNA, Viral/genetics/isolation & purification
MH  - Restriction Mapping
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
EDAT- 2003/06/27 05:00
MHDA- 2003/09/25 05:00
CRDT- 2003/06/27 05:00
PHST- 2003/06/27 05:00 [pubmed]
PHST- 2003/09/25 05:00 [medline]
PHST- 2003/06/27 05:00 [entrez]
AID - 10.1007/s00705-003-0014-z [doi]
PST - ppublish
SO  - Arch Virol. 2003 Jul;148(7):1387-403. doi: 10.1007/s00705-003-0014-z.