PMID- 12827471
OWN - NLM
STAT- MEDLINE
DCOM- 20030923
LR  - 20131121
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 148
IP  - 7
DP  - 2003 Jul
TI  - A putative DNA helicase and novel oligoribonuclease in the Diachasmimorpha 
      longicaudata entomopoxvirus (DlEPV).
PG  - 1431-44
AB  - Diachasmimorpha longicaudata entomopoxvirus (DlEPV) is a symbiotic entomopoxvirus 
      (EPV) of the parasitic wasp Diachasmimorpha longicaudata. It has a 
      double-stranded DNA genome of 250-300 kb and is >60% A-T rich. We describe ten 
      ORFs (RI-35-1 to -10) contained within a 5.64 kb clone, RI-35, from a DlEPV EcoRI 
      genomic library. Our goal was to identify unique motifs and compare them with 
      others in the database, particularly those of poxviruses. Two ORFs (RI-35-1 and 
      RI-35-7, respectively) encode putative proteins (113 aa and 219 aa) that are 
      probably involved in regulating gene expression based on their predicted nuclear 
      localization and the presence of SPxx motifs, leucine-zipper like sequences (113 
      aa), and a basic domain (219 aa). The largest gene (RI-35-3) is under the control 
      of an intermediate/late promoter and is presumed to encode a cytoplasmic 480 aa 
      DNA-dependent DNA helicase with conserved motifs that are characteristic of DExH 
      helicases. Amino acid analysis of the DNA helicase sequence showed that DlEPV is 
      close to but distinct from the Genus B EPVs. The DlEPV helicase is also distinct 
      from that of the Diadromus pulchellus ascovirus 1a from the D. pulchellus 
      parasitic wasp, with less than 10% amino acid identity. DlEPV encodes a 207 aa 
      oligoribonuclease (RI-35-8) of the DEDDh family of exoribonucleases. The second 
      largest ORF (RI-35-9) is under the control of a poxvirus early promoter and 
      encodes a protein of 329 aa that is likely DlEPV-specific. Three ORFs (RI-35-4, 
      -5, and -6) overlap (in the anti-sense strand) with ORFs encoding putatively 
      important virus replication proteins (which were also under the control of 
      intermediate promoters) and are presumably not expressed in DlEPV. These results 
      support earlier reports that DlEPV is a member of the sub-family 
      Entomopoxvirinae, most likely in Group C, and is the first symbiotic EPV 
      described to date from a parasitic wasp.
FAU - Mwaengo, D M
AU  - Mwaengo DM
AD  - Department of Entomology and Nematology, University of Florida, Gainesville, 
      Florida 32611-0620, USA.
FAU - Lawrence, P O
AU  - Lawrence PO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (DNA, Viral)
RN  - 0 (Peptides)
RN  - 0 (Viral Proteins)
RN  - EC 3.1.- (Exoribonucleases)
RN  - EC 3.1.- (oligoribonuclease)
RN  - EC 3.6.4.- (DNA Helicases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - DNA Helicases/chemistry/genetics/*metabolism
MH  - DNA, Viral/genetics
MH  - Drosophila melanogaster/genetics
MH  - Entomopoxvirinae/*enzymology/*genetics
MH  - Exoribonucleases/chemistry/genetics/*metabolism
MH  - *Genome, Viral
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Peptides/chemistry/genetics
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Symbiosis
MH  - Viral Proteins/chemistry/genetics
MH  - Wasps/virology
EDAT- 2003/06/27 05:00
MHDA- 2003/09/25 05:00
CRDT- 2003/06/27 05:00
PHST- 2003/06/27 05:00 [pubmed]
PHST- 2003/09/25 05:00 [medline]
PHST- 2003/06/27 05:00 [entrez]
AID - 10.1007/s00705-002-0975-3 [doi]
PST - ppublish
SO  - Arch Virol. 2003 Jul;148(7):1431-44. doi: 10.1007/s00705-002-0975-3.