PMID- 1282887
OWN - NLM
STAT- MEDLINE
DCOM- 19930218
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 210
IP  - 3
DP  - 1992 Dec 15
TI  - A mutant of human insulin-like growth factor II (IGF II) with the processing 
      sites of proinsulin. Expression and binding studies of processed IGF II.
PG  - 665-9
AB  - A mutant of human insulin-like growth factor II (IGF II) was constructed by 
      site-directed mutagenesis: the nucleotides coding for Ser33 and Ser39 were 
      changed to yield Arg and Lys, respectively, thus creating two pairs of basic 
      residues, Arg-Arg and Lys-Arg, as flanking sequences of the remaining C domain. 
      [Arg33, Lys39]IGF II was expressed in NIH-3T3 cells as a processed two-chain 
      peptide with a deletion of amino acid residues 37-40 and crosslinked by three 
      disulfide bonds. This des(37-40)[Arg33]IGF II showed 3.6-fold and 7.4-fold 
      reduced affinities to the type 1 and type 2 IGF receptor overexpressing cells, 
      respectively, whereas the thymidine incorporation potency was the same as that of 
      wild-type IGF II. We speculate that the discrepancy between the reduced binding 
      to the type 1 IGF receptor and the full thymidine incorporation potency is due to 
      the 6.1-fold reduced affinity of the expressed mutant to the co-expressed IGF 
      binding protein 3 (IGFBP-3). The results suggest that des(37-40)[Arg33]IGF II 
      assumes a conformation very similar to IGF II, and that the entire length of the 
      C domain is not essential for biological activity.
FAU - Zarn, J A
AU  - Zarn JA
AD  - Department of Biochemistry, University of Zurich, Switzerland.
FAU - Luthi, C
AU  - Luthi C
FAU - Giger, R J
AU  - Giger RJ
FAU - Sigrist, A
AU  - Sigrist A
FAU - Humbel, R E
AU  - Humbel RE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Carrier Proteins)
RN  - 0 (Insulin-Like Growth Factor Binding Protein 2)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Receptor, IGF Type 2)
RN  - 0 (Recombinant Proteins)
RN  - 67763-97-7 (Insulin-Like Growth Factor II)
RN  - 9035-68-1 (Proinsulin)
RN  - EC 2.7.10.1 (Receptor, IGF Type 1)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carrier Proteins/metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Humans
MH  - Insulin-Like Growth Factor Binding Protein 2
MH  - Insulin-Like Growth Factor II/*genetics/isolation & purification/metabolism
MH  - Kinetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Mutagenesis, Site-Directed
MH  - Oligodeoxyribonucleotides
MH  - Proinsulin/*genetics
MH  - Receptor, IGF Type 1/metabolism
MH  - Receptor, IGF Type 2/metabolism
MH  - Recombinant Proteins/isolation & purification/metabolism
MH  - Restriction Mapping
MH  - Transfection
EDAT- 1992/12/15 00:00
MHDA- 1992/12/15 00:01
CRDT- 1992/12/15 00:00
PHST- 1992/12/15 00:00 [pubmed]
PHST- 1992/12/15 00:01 [medline]
PHST- 1992/12/15 00:00 [entrez]
AID - 10.1111/j.1432-1033.1992.tb17467.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1992 Dec 15;210(3):665-9. doi: 10.1111/j.1432-1033.1992.tb17467.x.