PMID- 12830459
OWN - NLM
STAT- MEDLINE
DCOM- 20040325
LR  - 20190815
IS  - 1523-6838 (Electronic)
IS  - 0272-6386 (Linking)
VI  - 42
IP  - 1
DP  - 2003 Jul
TI  - Detection of nuclear factor-kappaB in IgA nephropathy using Southwestern 
      histochemistry.
PG  - 76-86
AB  - BACKGROUND: The transcription factor nuclear factor-kappaB (NF-kappaB) is 
      involved in inflammatory and immune responses through induction of various 
      cytokines and growth factors. The aim of this study is to examine the correlation 
      between NF-kappaB expression and severity of tissue injury in immunoglobulin A 
      (IgA) nephropathy and the mechanism of such correlation. METHODS: The study 
      included 43 renal tissue samples from 28 patients, including 28 samples of IgA 
      nephropathy, 5 samples of non-IgA mesangial proliferative glomerulonephritis 
      (non-IgA nephropathy), and 10 samples with nonproliferative glomerulonephritis 
      (membranous nephropathy [MN] n = 5; minimal change nephrotic syndrome [MCNS]; n = 
      5). Tissue sections were examined by Southwestern histochemistry and 
      immunohistochemistry for monocyte chemoattractant protein-1 (MCP-1), 
      granulocyte-macrophage colony-stimulating factor (GM-CSF), and intercellular cell 
      adhesion molecule-1 (ICAM-1), which are regulated by NF-kappaB. Normal portions 
      of surgically resected kidney with adenocarcinoma served as controls. RESULTS: In 
      normal kidney, MCNS, and MN sections, NF-kappaB expression was detected in a few 
      mesangial cells and tubular epithelial cells. In IgA nephropathy and non-IgA 
      nephropathy samples, NF-kappaB was expressed in mesangial, glomerular endothelial 
      and epithelial cells, tubular epithelial cells, and infiltrating cells. 
      Expression in both glomeruli and interstitium correlated with progression of 
      tissue injury. In IgA nephropathy samples, MCP-1 and GM-CSF expression was 
      increased in both glomeruli and interstitium and correlated with progression of 
      tissue injury. Glomerular ICAM-1 expression was weaker in severe lesions, whereas 
      interstitial expression correlated with progression of tissue injury. CONCLUSION: 
      Our results indicate that NF-kappaB is involved in the progression of tissue 
      injury in IgA nephropathy through the induction of transcriptionally regulated 
      genes.
FAU - Ashizawa, Mamiko
AU  - Ashizawa M
AD  - Second Department of Internal Medicine, Division of Nephrology, Nagasaki 
      University School of Medicine, Nagasaki, Japan.
FAU - Miyazaki, Masanobu
AU  - Miyazaki M
FAU - Abe, Katsushige
AU  - Abe K
FAU - Furusu, Akira
AU  - Furusu A
FAU - Isomoto, Hajime
AU  - Isomoto H
FAU - Harada, Takashi
AU  - Harada T
FAU - Ozono, Yoshiyuki
AU  - Ozono Y
FAU - Sakai, Hideto
AU  - Sakai H
FAU - Koji, Takehiko
AU  - Koji T
FAU - Kohno, Shigeru
AU  - Kohno S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Kidney Dis
JT  - American journal of kidney diseases : the official journal of the National Kidney 
      Foundation
JID - 8110075
RN  - 0 (Chemokine CCL2)
RN  - 0 (NF-kappa B)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Adenocarcinoma/metabolism/pathology
MH  - Adult
MH  - *Blotting, Southwestern
MH  - Chemokine CCL2/analysis
MH  - Disease Progression
MH  - Endothelial Cells/metabolism/ultrastructure
MH  - Epithelial Cells/metabolism/ultrastructure
MH  - Female
MH  - Gene Expression Regulation
MH  - Glomerulonephritis, IGA/genetics/*metabolism/pathology
MH  - Glomerulonephritis, Membranous/genetics/metabolism/pathology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/analysis
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/analysis
MH  - Kidney/*metabolism/pathology
MH  - Kidney Glomerulus/metabolism/ultrastructure
MH  - Kidney Neoplasms/metabolism/pathology
MH  - Kidney Tubules/metabolism/ultrastructure
MH  - Male
MH  - Middle Aged
MH  - NF-kappa B/*analysis
MH  - Nephrosis, Lipoid/genetics/metabolism/pathology
MH  - Single-Blind Method
MH  - Transcription, Genetic
EDAT- 2003/06/28 05:00
MHDA- 2004/03/26 05:00
CRDT- 2003/06/28 05:00
PHST- 2003/06/28 05:00 [pubmed]
PHST- 2004/03/26 05:00 [medline]
PHST- 2003/06/28 05:00 [entrez]
AID - S0272638603004116 [pii]
AID - 10.1016/s0272-6386(03)00411-6 [doi]
PST - ppublish
SO  - Am J Kidney Dis. 2003 Jul;42(1):76-86. doi: 10.1016/s0272-6386(03)00411-6.