PMID- 12831775
OWN - NLM
STAT- MEDLINE
DCOM- 20040317
LR  - 20190916
IS  - 1096-4959 (Print)
IS  - 1096-4959 (Linking)
VI  - 135
IP  - 3
DP  - 2003 Jul
TI  - Induction of vitellogenesis by estradiol-17beta and development of enzyme-linked 
      immunosorbant assays to quantify plasma vitellogenin levels in green turtles 
      (Chelonia mydas).
PG  - 551-63
AB  - Treatment of juvenile green turtles (Chelonia mydas) with estradiol-17beta 
      resulted in the induction of a 200 kDa plasma protein, consistent with 
      vitellogenin (Vtg). The N-terminal 15 amino acids of the anion exchange purified 
      protein shared sequence homologies with vitellogenins of several vertebrate 
      species. Rabbit antiserum raised against purified Vtg recognized the plasma 
      protein as well as several yolk proteins. Monoclonal antibody (Mab) HL1248, 
      produced by inoculating mice with turtle yolk granules, showed specificity for 
      plasma Vtg as well as a set of yolk proteins 120, 82, 43 and 32 kDa in size. The 
      N-terminal 22 amino acids of the 43 kDa yolk protein was similar to the 
      lipovitellin I subunit of Vtg of several vertebrate species. The peptide mass map 
      of the 82 kDa yolk protein shared enough ions with that of purified plasma Vtg to 
      support the conclusion that this protein was derived from plasma Vtg. Taken 
      together, these results validate the specificity of Mab HL1248 for Vtg. Using 
      purified Vtg concentration standards, competition and antigen capture 
      enzyme-linked immunosorbant assays (ELISAs) were shown to quantitatively detect 
      Vtg in green turtle plasma. Pre-induced plasma of juvenile turtles had Vtg levels 
      of 2-4 micrograms/ml whereas post-estradiol exposure samples had 38-40 mg/ml. The 
      plasma Vtg concentration of a nesting female turtle was 4.6 mg/ml, approximately 
      20-fold higher than that of a non-nesting adult female. The antigen capture ELISA 
      will be useful in population studies of this endangered species, to detect 
      vitellogenesis in females that will nest in a given year and to detect 
      inappropriate Vtg levels in turtles exposed to xenoestrogens.
FAU - Herbst, Lawrence H
AU  - Herbst LH
AD  - Department of Pathology, Albert Einstein College of Medicine, 10461, Bronx, NY, 
      USA. herbst@aecom.yu.edu
FAU - Siconolfi-Baez, Linda
AU  - Siconolfi-Baez L
FAU - Torelli, John H
AU  - Torelli JH
FAU - Klein, Paul A
AU  - Klein PA
FAU - Kerben, Marci J
AU  - Kerben MJ
FAU - Schumacher, Isabella M
AU  - Schumacher IM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Comp Biochem Physiol B Biochem Mol Biol
JT  - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
JID - 9516061
RN  - 0 (Antibodies)
RN  - 0 (Egg Proteins)
RN  - 0 (Vitellogenins)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies/immunology
MH  - Egg Proteins/blood/immunology
MH  - Egg Yolk/chemistry
MH  - Enzyme-Linked Immunosorbent Assay/instrumentation/*methods
MH  - Estradiol/*pharmacology
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - Turtles/*blood
MH  - Vitellogenesis/*drug effects
MH  - Vitellogenins/*blood/immunology
EDAT- 2003/07/02 05:00
MHDA- 2004/03/18 05:00
CRDT- 2003/07/02 05:00
PHST- 2003/07/02 05:00 [pubmed]
PHST- 2004/03/18 05:00 [medline]
PHST- 2003/07/02 05:00 [entrez]
AID - S1096495903001416 [pii]
AID - 10.1016/s1096-4959(03)00141-6 [doi]
PST - ppublish
SO  - Comp Biochem Physiol B Biochem Mol Biol. 2003 Jul;135(3):551-63. doi: 
      10.1016/s1096-4959(03)00141-6.