PMID- 12831860
OWN - NLM
STAT- MEDLINE
DCOM- 20030905
LR  - 20190712
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 119
IP  - 4
DP  - 2003
TI  - Dark-rearing decreases NR2A N-methyl-D-aspartate receptor subunit in all visual 
      cortical layers.
PG  - 1013-22
AB  - Maturation of the visual cortex is a visual experience-dependent process. It has 
      been shown that visual input triggers changes in N-methyl-D-aspartate receptor 
      (NMDAR) subunit expression in the visual cortex. However, no data are available 
      on the layer distribution of these molecular changes. Here we describe the 
      laminar distribution of the cells expressing the NMDAR subunits NR2A and NR2B in 
      the rat primary visual cortex at postnatal day (P) 21 and 37 using anti-NR2A and 
      anti-NR2B antibodies and a stereological method to count labelled neurons. The 
      percentage of neurons expressing the NR2A subunit in the layers II-VI remained 
      unchanged between P21 and P37 with a slight decrease in layer V. Dark-rearing 
      from P21 to P37 induced a pronounced decrease of the staining intensity and a 
      significant decrease in the percentage of NR2A-expressing neurons. The changes in 
      NR2A expression caused by dark rearing occur at similar levels in layers II-VI. 
      The percentage of NR2B-positive cells in the different cortical layers remains 
      unchanged from P21 to P37. The NR2B pattern was not significantly affected by 
      dark-rearing. Thusly, the expression of NR2A depends upon visual experience after 
      P21.
FAU - Tongiorgi, E
AU  - Tongiorgi E
AD  - BRAIN Centre for Neuroscience, Department of Biology, University of Trieste, 
      34127, Trieste, Italy.
FAU - Ferrero, F
AU  - Ferrero F
FAU - Cattaneo, A
AU  - Cattaneo A
FAU - Domenici, L
AU  - Domenici L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (NR2A NMDA receptor)
RN  - 0 (NR2B NMDA receptor)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
SB  - IM
MH  - Aging/metabolism
MH  - Animals
MH  - Cell Count
MH  - Cell Differentiation/physiology
MH  - Dark Adaptation/*physiology
MH  - Down-Regulation/*physiology
MH  - Immunohistochemistry
MH  - Neurons/cytology/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Sensory Deprivation/*physiology
MH  - Synaptic Transmission/physiology
MH  - Visual Cortex/cytology/*growth & development/*metabolism
MH  - Visual Perception/physiology
EDAT- 2003/07/02 05:00
MHDA- 2003/09/06 05:00
CRDT- 2003/07/02 05:00
PHST- 2003/07/02 05:00 [pubmed]
PHST- 2003/09/06 05:00 [medline]
PHST- 2003/07/02 05:00 [entrez]
AID - S0306452203001969 [pii]
AID - 10.1016/s0306-4522(03)00196-9 [doi]
PST - ppublish
SO  - Neuroscience. 2003;119(4):1013-22. doi: 10.1016/s0306-4522(03)00196-9.