PMID- 12834432
OWN - NLM
STAT- MEDLINE
DCOM- 20030904
LR  - 20190922
IS  - 0953-8194 (Print)
IS  - 0953-8194 (Linking)
VI  - 15
IP  - 8
DP  - 2003 Aug
TI  - Circulating angiotensin II activates neurones in circumventricular organs of the 
      lamina terminalis that project to the bed nucleus of the stria terminalis.
PG  - 725-31
AB  - The aim of this study was to determine, in conscious rats, whether elevated 
      concentrations of circulating angiotensin II activate neurones in both the 
      subfornical organ and organum vasculosum of the lamina terminalis (OVLT) that 
      project to the bed nucleus of the stria terminalis (BNST). The strategy employed 
      was to colocalize retrogradely transported cholera toxin B subunit (CTB) from the 
      BNST, with elevated levels of Fos protein in response to angiotensin II. 
      Circulating angiotensin II concentrations were increased by either intravenous 
      infusion of angiotensin II or subcutaneous injection of isoproterenol. Neurones 
      exhibiting Fos in response to angiotensin II were present in the subfornical 
      organ, predominantly in its central core but with some also seen in its 
      peripheral aspect, the dorsal and lateral margins of the OVLT, the supraoptic 
      nucleus and the parvo- and magnocellular divisions of the paraventricular 
      nucleus. Fos-labelling was not apparent in control rats infused with isotonic 
      saline intravenously or injected with either CTB or CTB conjugated to gold 
      particles (CTB-gold) only. Of the neurones in the subfornical organ that were 
      shown by retrograde labelling to project to BNST, approximately 50% expressed Fos 
      in response to isoproterenol. This stimulus also increased Fos in 33% of neurones 
      in the OVLT that project to BNST. Double-labelled neurones were concentrated in 
      the central core of the subfornical organ and lateral margins of the OVLT in 
      response to increased circulating angiotensin II resulting from isoproterenol 
      treatment. These data support a role for circulating angiotensin II acting either 
      directly or indirectly on neurones in subfornical organ and OVLT that project to 
      the BNST and provide further evidence of functional regionalization within the 
      subfornical organ and the OVLT. The function of these pathways is yet to be 
      determined; however, a role in body fluid homeostasis is possible.
FAU - Sunn, N
AU  - Sunn N
AD  - Howard Florey Institute of Experimental Physiology and Medicine, University of 
      Melbourne, Melbourne, Victoria, Australia.
FAU - McKinley, M J
AU  - McKinley MJ
FAU - Oldfield, B J
AU  - Oldfield BJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neuroendocrinol
JT  - Journal of neuroendocrinology
JID - 8913461
RN  - 0 (Isotonic Solutions)
RN  - 0 (Sympathomimetics)
RN  - 0 (Vasoconstrictor Agents)
RN  - 11128-99-7 (Angiotensin II)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 9012-63-9 (Cholera Toxin)
RN  - EC 3.4.23.15 (Renin)
RN  - L628TT009W (Isoproterenol)
SB  - IM
MH  - Angiotensin II/*blood/pharmacology
MH  - Animals
MH  - Cholera Toxin/pharmacology
MH  - Drinking/physiology
MH  - Femoral Vein
MH  - Hypothalamus/cytology/*physiology
MH  - Infusions, Intravenous
MH  - Injections, Subcutaneous
MH  - Isoproterenol/pharmacology
MH  - Isotonic Solutions/pharmacology
MH  - Kidney/metabolism
MH  - Male
MH  - Neural Pathways
MH  - Neurons/drug effects/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Renin/metabolism
MH  - Septal Nuclei/cytology/*physiology
MH  - Sodium Chloride/pharmacology
MH  - Sympathomimetics/pharmacology
MH  - Vasoconstrictor Agents/*blood/pharmacology
EDAT- 2003/07/02 05:00
MHDA- 2003/09/05 05:00
CRDT- 2003/07/02 05:00
PHST- 2003/07/02 05:00 [pubmed]
PHST- 2003/09/05 05:00 [medline]
PHST- 2003/07/02 05:00 [entrez]
AID - 969 [pii]
AID - 10.1046/j.1365-2826.2003.00969.x [doi]
PST - ppublish
SO  - J Neuroendocrinol. 2003 Aug;15(8):725-31. doi: 10.1046/j.1365-2826.2003.00969.x.