PMID- 12834847
OWN - NLM
STAT- MEDLINE
DCOM- 20041106
LR  - 20190901
IS  - 0301-4622 (Print)
IS  - 0301-4622 (Linking)
VI  - 104
IP  - 1
DP  - 2003 May 1
TI  - Epoxidation of styrene by human cyt P450 1A2 by thin film electrolysis and 
      peroxide activation compared to solution reactions.
PG  - 291-6
AB  - Films of human cytochrome P450 1A2 (cyt P450 1A2) and polystyrene sulfonate were 
      constructed on carbon cloth electrodes using layer-by-layer alternate absorption 
      and evaluated for electrochemical- and H(2)O(2)-driven enzyme-catalyzed oxidation 
      of styrene to styrene oxide. At -0.6 V vs. saturated calomel reference electrode 
      in an electrochemical cell, epoxidation of styrene was mediated by initial 
      catalytic reduction of dioxygen to H(2)O(2) which activates the enzyme for the 
      catalytic oxidation. Slightly larger turnover rates for cyt P450 1A2 were found 
      for the electrolytic and H(2)O(2) (10 mM) driven reactions compared to 
      conventional enzymatic reactions using cyt P450s, reductases, and electron donors 
      for cytochromes P450 1A2. Cyt P450(cam) gave comparable turnover rates in film 
      electrolysis and solution reactions. Results demonstrate that cyt P450 1A2 
      catalyzes styrene epoxidation faster than cyt P450(cam), and suggests the 
      usefulness of this thin-film electrolytic method for relative turnover rate 
      studies of cyt P450s.
FAU - Estavillo, Carmelita
AU  - Estavillo C
AD  - Department of Chemistry, U-3060, University of Connecticut, Storrs, CT 06269, 
      USA.
FAU - Lu, Zhongqing
AU  - Lu Z
FAU - Jansson, Ingela
AU  - Jansson I
FAU - Schenkman, John B
AU  - Schenkman JB
FAU - Rusling, James F
AU  - Rusling JF
LA  - eng
GR  - ES03154/ES/NIEHS NIH HHS/United States
PT  - Letter
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biophys Chem
JT  - Biophysical chemistry
JID - 0403171
RN  - 0 (Epoxy Compounds)
RN  - 0 (Solutions)
RN  - 44LJ2U959V (Styrene)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A2)
SB  - IM
MH  - Animals
MH  - Cytochrome P-450 CYP1A2/*chemistry/metabolism
MH  - Electrochemistry
MH  - Electrodes
MH  - Epoxy Compounds/*chemistry
MH  - Humans
MH  - Hydrogen Peroxide/*chemistry
MH  - Oxidation-Reduction
MH  - Rats
MH  - Solutions/chemistry
MH  - Styrene/*chemistry
EDAT- 2003/07/02 05:00
MHDA- 2004/11/09 09:00
CRDT- 2003/07/02 05:00
PHST- 2003/07/02 05:00 [pubmed]
PHST- 2004/11/09 09:00 [medline]
PHST- 2003/07/02 05:00 [entrez]
AID - S0301462202003836 [pii]
AID - 10.1016/s0301-4622(02)00383-6 [doi]
PST - ppublish
SO  - Biophys Chem. 2003 May 1;104(1):291-6. doi: 10.1016/s0301-4622(02)00383-6.