PMID- 12834849
OWN - NLM
STAT- MEDLINE
DCOM- 20041106
LR  - 20190901
IS  - 0301-4622 (Print)
IS  - 1873-4200 (Electronic)
IS  - 0301-4622 (Linking)
VI  - 104
IP  - 1
DP  - 2003 May 1
TI  - Interactions of chlorophyllin with acridine orange, quinacrine mustard and 
      doxorubicin analyzed by light absorption and fluorescence spectroscopy.
PG  - 305-13
AB  - The present study was designed to estimate the ability of chlorophyllin (CHL) to 
      interact with two acridine mutagens, quinacrine mustard (QM) and acridine orange 
      (AO), and with the antitumor anthracycline doxorubicin (Dox). To this end, 
      aqueous solutions of QM, AO or Dox during titration with CHL were subjected to 
      spectrophotometry and spectrofluorimetry to detect possible interactions between 
      these reagents. The data indicate that CHL forms complexes with AO, QM or Dox in 
      these solutions. The presence of the complexes was manifested by a bathochromic 
      shift of the absorption spectra, as well as by strong quenching of the 
      fluorescence of each of these mutagens in the presence of CHL. CHL, thus, may 
      serve as an interceptor of these mutagenic acridines in different in vivo or in 
      vitro applications. Its ability to interact with Dox may potentially be utilized 
      to detoxify patients overdosed with this or similar drugs.
FAU - Pietrzak, Monika
AU  - Pietrzak M
AD  - Department of Physics and Biophysics, University of Warmia and Mazury in Olsztyn, 
      Oczapowskiego 4, 10-719 Olsztyn, Poland.
FAU - Wieczorek, Zbigniew
AU  - Wieczorek Z
FAU - Stachelska, Alicja
AU  - Stachelska A
FAU - Darzynkiewicz, Zbigniew
AU  - Darzynkiewicz Z
LA  - eng
GR  - R01 CA028704/CA/NCI NIH HHS/United States
GR  - R01 CA028704-29/CA/NCI NIH HHS/United States
GR  - R01 CA28704/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biophys Chem
JT  - Biophysical chemistry
JID - 0403171
RN  - 0 (Antimutagenic Agents)
RN  - 0 (Chlorophyllides)
RN  - 0 (Mutagens)
RN  - 4213-45-0 (Quinacrine Mustard)
RN  - 80168379AG (Doxorubicin)
RN  - EEM82VOY7C (chlorophyllin)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/chemistry/*metabolism
MH  - Antimutagenic Agents/*chemistry
MH  - Chlorophyllides/*chemistry
MH  - Dose-Response Relationship, Drug
MH  - Doxorubicin/*chemistry/metabolism
MH  - Humans
MH  - Molecular Structure
MH  - Mutagens/*chemistry/metabolism
MH  - Quinacrine Mustard/*chemistry/metabolism
MH  - Spectrometry, Fluorescence
MH  - Spectrophotometry
PMC - PMC3480723
MID - NIHMS76822
EDAT- 2003/07/02 05:00
MHDA- 2004/11/09 09:00
PMCR- 2012/10/25
CRDT- 2003/07/02 05:00
PHST- 2003/07/02 05:00 [pubmed]
PHST- 2004/11/09 09:00 [medline]
PHST- 2003/07/02 05:00 [entrez]
PHST- 2012/10/25 00:00 [pmc-release]
AID - S0301462202003873 [pii]
AID - 10.1016/s0301-4622(02)00387-3 [doi]
PST - ppublish
SO  - Biophys Chem. 2003 May 1;104(1):305-13. doi: 10.1016/s0301-4622(02)00387-3.