PMID- 12835098
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20031112
LR  - 20230320
IS  - 1029-8428 (Print)
IS  - 1029-8428 (Linking)
VI  - 1
IP  - 4
DP  - 2000 Apr
TI  - BDNF is involved in sympathetic sprouting in the dorsal root ganglia following 
      peripheral nerve injury in rats.
PG  - 311-22
AB  - Peripheral nerve injury results in sympathetic sprouting around large diameter 
      sensory neurons in the dorsal root ganglia (DRG). The mechanism underlying this 
      pathological phenomenon is not known. Brain-derived neurotrophic factor (BDNF) is 
      up-regulated in large sensory neurons and ensheathing satellite cells following a 
      sciatic nerve injury. In the present study, we investigated the effects of BDNF 
      on the sympathetic sprouting in the DRG, by delivering BDNF antibody or antisense 
      oligodeoxynucleotide to injured DRGs, or by delivering exogenous BDNF to intact 
      DRGs. The sheep antibody to BDNF, characterized by bioassays and dot blots, 
      specifically reacted with BDNF but not other neurotrophins. Noradrenergic fibers 
      were visualized by immunostaining of tyrosine hydroxylase (TH) and quantified by 
      an NIH Imaging program. Two weeks following L5 spinal nerve lesion, a dramatic 
      increase in TH-immunoreactive (-ir) fibres was observed in both ipsi- and 
      contralateral DRGs in normal sheep IgG treated rats. BDNF antibody significantly 
      reduced the sprouting of sympathetic nerves in both ipsi- and contra-lateral DRGs 
      by 67% and 42% respectively. BDNF antisense oligodeoxynucleotide, by inhibiting 
      BDNF synthesis in DRGs, also significantly suppressed the sprouting by 67% and 
      60% respectively in the ipsi- and contra-lateral DRGs. Delivery of exogenous BDNF 
      into an intact L5 DRGs resulted in an increase in the sprouting by 4.2-fold. Our 
      results clearly indicate that BDNF, synthesized in and secreted from the DRGs, is 
      involved in the sympathetic sprouting in the DRG following the peripheral nerve 
      injury.
FAU - Deng, Y S
AU  - Deng YS
AD  - Department of Human Physiology and Center for Neuroscience, Flinders University 
      of South Australia, GPO Box 2100, Adelaide 5001, Australia.
FAU - Zhong, J H
AU  - Zhong JH
FAU - Zhou, X F
AU  - Zhou XF
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Neurotox Res
JT  - Neurotoxicity research
JID - 100929017
EDAT- 2003/07/02 05:00
MHDA- 2003/07/02 05:01
CRDT- 2003/07/02 05:00
PHST- 2003/07/02 05:00 [pubmed]
PHST- 2003/07/02 05:01 [medline]
PHST- 2003/07/02 05:00 [entrez]
AID - 10.1007/BF03033260 [doi]
PST - ppublish
SO  - Neurotox Res. 2000 Apr;1(4):311-22. doi: 10.1007/BF03033260.