PMID- 12835757
OWN - NLM
STAT- MEDLINE
DCOM- 20040420
LR  - 20200930
IS  - 1469-221X (Print)
IS  - 1469-3178 (Electronic)
IS  - 1469-221X (Linking)
VI  - 4
IP  - 7
DP  - 2003 Jul
TI  - A targeted deletion in the endocytic receptor gene Endo180 results in a defect in 
      collagen uptake.
PG  - 710-6
AB  - The four members of the mannose receptor family (the mannose receptor, the M-type 
      phospholipase A(2) receptor, DEC-205 and Endo180) share a common extracellular 
      arrangement of an amino-terminal cysteine-rich domain followed by a fibronectin 
      type II (FNII) domain and multiple C-type lectin-like domains (CTLDs). In 
      addition, all have a short cytoplasmic domain, which mediates their constitutive 
      recycling between the plasma membrane and the endosomal apparatus, suggesting 
      that these receptors function to internalize ligands for intracellular delivery. 
      We have generated mice with a targeted deletion of Endo180 exons 2-6 and show 
      that this mutation results in the efficient expression of a truncated Endo180 
      protein that lacks the cysteine-rich domain, the FNII domain and CTLD1. Analysis 
      of embryonic fibroblasts reveals that this mutation does not disrupt the C-type 
      lectin activity that is mediated by CTLD2, but results in cells that have a 
      defect in collagen binding and internalization and an impaired migratory 
      phenotype.
FAU - East, Lucy
AU  - East L
AD  - The Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, 237 
      Fulham Road, London SW3 6JB, UK.
FAU - McCarthy, Afshan
AU  - McCarthy A
FAU - Wienke, Dirk
AU  - Wienke D
FAU - Sturge, Justin
AU  - Sturge J
FAU - Ashworth, Alan
AU  - Ashworth A
FAU - Isacke, Clare M
AU  - Isacke CM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO Rep
JT  - EMBO reports
JID - 100963049
RN  - 0 (Endo180)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Ligands)
RN  - 0 (Receptors, Mitogen)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Collagen/*metabolism
MH  - Cytoplasm/metabolism
MH  - Fibroblasts
MH  - Gene Expression Regulation
MH  - Lectins, C-Type/chemistry/genetics/metabolism
MH  - Ligands
MH  - Mice
MH  - Protein Binding
MH  - Protein Transport
MH  - Receptors, Mitogen/chemistry/*genetics/*metabolism
MH  - Sequence Deletion/*genetics
PMC - PMC1326321
EDAT- 2003/07/02 05:00
MHDA- 2004/04/21 05:00
PMCR- 2004/07/01
CRDT- 2003/07/02 05:00
PHST- 2003/02/21 00:00 [received]
PHST- 2003/05/07 00:00 [revised]
PHST- 2003/05/13 00:00 [accepted]
PHST- 2003/07/02 05:00 [pubmed]
PHST- 2004/04/21 05:00 [medline]
PHST- 2003/07/02 05:00 [entrez]
PHST- 2004/07/01 00:00 [pmc-release]
AID - embor882 [pii]
AID - 10.1038/sj.embor.embor882 [doi]
PST - ppublish
SO  - EMBO Rep. 2003 Jul;4(7):710-6. doi: 10.1038/sj.embor.embor882.