PMID- 12836006
OWN - NLM
STAT- MEDLINE
DCOM- 20030930
LR  - 20200930
IS  - 0721-7714 (Print)
IS  - 0721-7714 (Linking)
VI  - 21
IP  - 11
DP  - 2003 Jul
TI  - Long-term stability of transgene expression driven by barley endosperm-specific 
      hordein promoters in transgenic barley.
PG  - 1108-20
AB  - In order to evaluate the long-term stability of transgene expression driven by 
      the B(1)- and D-hordein promoters in transgenic barley ( Hordeum vulgare L., 2 
      n=2 x=14), we analyzed plants from 15 independent transgenic barley lines [6 for 
      uidA and 9 for sgfp(S65T)] produced via microprojectile bombardment of immature 
      embryos; 4 were diploid and 11 were tetraploid. The expression and inheritance of 
      transgenes were determined by analysis of functional transgene expression, 
      polymerase chain reaction and fluorescence in situ hybridization (FISH). Ability 
      to express transgenes driven by either B(1)- or D-hordein promoter was inherited 
      in T(4) and later generations: T(4) (2 lines), T(5) (8 lines), T(6) (3 lines), 
      T(8) (1 line) and T(9) (1 line). Homozygous transgenic plants were obtained from 
      12 lines [5 for uidA and 7 for sgfp(S65T)]; the remaining lines are currently 
      being analyzed. The application of the FISH technique for physical mapping of 
      chromosomes was useful for early screening of homozygous plants by examining for 
      presence of the transgene. For example, one line expressing uidA, and shown to 
      have doublet fluorescence signals on a pair of homologous chromosomes was 
      confirmed as a homozygous line by its segregation ratio; additionally this line 
      showed stable inheritance of the transgene to T(9) progeny. The expression of 
      transgenes in most lines (14 out of 15 lines) driven by hordein promoters was 
      stably transmitted to T(4) or later generations, although there was a skewed 
      segregation pattern (1:1) from the T(1) generation onward in the remaining line. 
      In contrast, transgene silencing or transgene loss under the control of the maize 
      ubiquitin promoter was observed in progeny of only 6 out of 15 lines.
FAU - Choi, H W
AU  - Choi HW
AD  - Department of Plant and Microbial Biology, University of California, Berkeley, CA 
      94720, USA.
FAU - Lemaux, P G
AU  - Lemaux PG
FAU - Cho, M-J
AU  - Cho MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20030429
PL  - Germany
TA  - Plant Cell Rep
JT  - Plant cell reports
JID - 9880970
RN  - 0 (Plant Proteins)
RN  - 8002-80-0 (Glutens)
SB  - IM
MH  - Chromosome Mapping
MH  - *Gene Expression Regulation, Plant
MH  - Genes, Reporter/genetics
MH  - Glutens
MH  - Hordeum/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Plant Proteins/*genetics
MH  - Plants, Genetically Modified
MH  - Promoter Regions, Genetic/*genetics
MH  - Transgenes/*genetics
EDAT- 2003/07/02 05:00
MHDA- 2003/10/01 05:00
CRDT- 2003/07/02 05:00
PHST- 2002/09/04 00:00 [received]
PHST- 2003/02/27 00:00 [revised]
PHST- 2003/03/02 00:00 [accepted]
PHST- 2003/07/02 05:00 [pubmed]
PHST- 2003/10/01 05:00 [medline]
PHST- 2003/07/02 05:00 [entrez]
AID - 10.1007/s00299-003-0630-9 [doi]
PST - ppublish
SO  - Plant Cell Rep. 2003 Jul;21(11):1108-20. doi: 10.1007/s00299-003-0630-9. Epub 
      2003 Apr 29.