PMID- 12836336
OWN - NLM
STAT- MEDLINE
DCOM- 20030815
LR  - 20191107
IP  - 1
DP  - 2001
TI  - Characterization and formation mechanism of water-insoluble DNA-matrix induced by 
      UV irradiation.
PG  - 205-6
AB  - We have prepared water-insoluble and nuclease resistant DNA-matrixes by UV 
      irradiation. The UV-irradiated DNA-matrix could effectively accumulate and 
      condense harmful DNA-intercalating compounds, such as acridine orange (AO) and 
      ethidium bromide (EB), from diluted aqueous solutions. The binding constant of AO 
      and EB for UV-irradiated DNA were determined to be 1.0 (+/- 0.2) x 10(5) M-1 and 
      6.8 (+/- 0.3) x 10(4) M-1, respectively; values consisted with reported results 
      for non-irradiated DNA. In addition, the agarose gel electrophoresis and AFM 
      measurements indicate that DNA matrix forms an intermolecular cross-linking 
      structure with the radical reaction. The UV-irradiated DNA-matrixes have 
      potential uses as a biomaterial filter for the removal of harmful DNA 
      intercalating compounds.
FAU - Yamada, M
AU  - Yamada M
AD  - Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido 
      University, Sapporo 060-0810, Japan.
FAU - Satoh, S
AU  - Satoh S
FAU - Nomizu, M
AU  - Nomizu M
FAU - Ohkawa, K
AU  - Ohkawa K
FAU - Yamamoto, H
AU  - Yamamoto H
FAU - Nishi, N
AU  - Nishi N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Res Suppl
JT  - Nucleic acids research. Supplement (2001)
JID - 101169367
RN  - 0 (Intercalating Agents)
RN  - 059QF0KO0R (Water)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/metabolism
MH  - DNA/chemistry/metabolism/*radiation effects/ultrastructure
MH  - Intercalating Agents/*metabolism
MH  - Microscopy, Atomic Force
MH  - *Ultraviolet Rays
MH  - Water/chemistry
EDAT- 2003/07/03 05:00
MHDA- 2003/08/16 05:00
CRDT- 2003/07/03 05:00
PHST- 2003/07/03 05:00 [pubmed]
PHST- 2003/08/16 05:00 [medline]
PHST- 2003/07/03 05:00 [entrez]
AID - 10.1093/nass/1.1.205 [doi]
PST - ppublish
SO  - Nucleic Acids Res Suppl. 2001;(1):205-6. doi: 10.1093/nass/1.1.205.