PMID- 12836339
OWN - NLM
STAT- MEDLINE
DCOM- 20030815
LR  - 20201208
IP  - 1
DP  - 2001
TI  - Telomerase-inhibitory effects of the triphosphate derivatives of some 
      biologically active nucleosides.
PG  - 211-2
AB  - Telomerase is a ribonucleoprotein reverse transcriptase that uses its internal 
      RNA moiety as a template for synthesis of telomere repeats. To clarify the 
      susceptibility of telomerase to HIV-1 reverse transcriptase inhibitors (RT), we 
      investigated the inhibitory effects of 3'-azido-3'-deoxythymidine 5'-triphosphate 
      (AZTTP), which is known to be a potent HIV-1 RT inhibitor, and acyclovir 
      triphosphate (ACVTP). Lineweaver-Burk plot analyses showed that the inhibition 
      mode of these compounds was competitive with the substrate dNTP counterpart. 
      However, inhibition by AZTTP was weak (Ki = 15 microM, Km of dTTP = 7.1 microM). 
      Interestingly, ACVTP showed considerable inhibition. The Ki value of ACVTP was 
      5.0 microM, being smaller than the Km of dGTP (12 microM).
FAU - Yamaguchi, T
AU  - Yamaguchi T
AD  - Department of Biological Sciences, Biotechnology Research Center, Teikyo 
      University of Science and Technology, Uenohara, Yamanashi 409-0193, Japan.
FAU - Takayama, Y
AU  - Takayama Y
FAU - Saito, M
AU  - Saito M
FAU - Ishikawa, F
AU  - Ishikawa F
FAU - Saneyoshi, M
AU  - Saneyoshi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Res Suppl
JT  - Nucleic acids research. Supplement (2001)
JID - 101169367
RN  - 0 (Dideoxynucleotides)
RN  - 0 (Nucleosides)
RN  - 0 (Reverse Transcriptase Inhibitors)
RN  - 0 (Thymine Nucleotides)
RN  - 4B9XT59T7S (Zidovudine)
RN  - 66341-18-2 (acyclovir triphosphate)
RN  - 6RGF96R053 (zidovudine triphosphate)
RN  - EC 2.7.7.49 (Telomerase)
RN  - X4HES1O11F (Acyclovir)
SB  - IM
MH  - Acyclovir/*analogs & derivatives/chemistry/*pharmacology
MH  - Dideoxynucleotides
MH  - HeLa Cells
MH  - Humans
MH  - Nucleosides/chemistry
MH  - Reverse Transcriptase Inhibitors/chemistry/*pharmacology
MH  - Telomerase/*metabolism
MH  - Thymine Nucleotides/chemistry/*pharmacology
MH  - Zidovudine/*analogs & derivatives/chemistry/*pharmacology
EDAT- 2003/07/03 05:00
MHDA- 2003/08/16 05:00
CRDT- 2003/07/03 05:00
PHST- 2003/07/03 05:00 [pubmed]
PHST- 2003/08/16 05:00 [medline]
PHST- 2003/07/03 05:00 [entrez]
AID - 10.1093/nass/1.1.211 [doi]
PST - ppublish
SO  - Nucleic Acids Res Suppl. 2001;(1):211-2. doi: 10.1093/nass/1.1.211.