PMID- 12836344
OWN - NLM
STAT- MEDLINE
DCOM- 20030815
LR  - 20210705
IP  - 1
DP  - 2001
TI  - Binding specificities of the mismatch binding protein, MutS, to oligonucleotides 
      containing modified bases.
PG  - 221-2
AB  - We have studied the effects of DNA mismatch repair on mutagenesis induced by 
      nucleoside analogs. Among them, the mutagenic action of 
      3,4-dihydro-6H,8H-pyrimido[4,5-c][1,2]oxazin-7-one 2'-deoxyriboside (dP) showed 
      high susceptibility to the mismatch repair system, while mutagenesis by 
      N4-aminocytidine and N4-hydroxycytidine was only weakly affected. 2-Aminopurine 
      mutagenesis showed intermediate susceptibility. MutS protein specifically bound 
      to an oligonucleotide duplex containing a dP-dG pair, while the dP-dA pair was 
      bound only weakly. The binding to the dP-dG pair was as strong as binding to a 
      dA-dC mismatch. These specific binding properties can explain the effective 
      avoidance of dP-induced mutagenesis by the mismatch repair system. We have also 
      studied the effects of the repair system on mutagenesis induced by methylating 
      and ethylating agents.
FAU - Negishi, K
AU  - Negishi K
AD  - Gene Research Center, Okayama University.
FAU - Maehara, D
AU  - Maehara D
FAU - Nakamura, S
AU  - Nakamura S
FAU - Loakes, D
AU  - Loakes D
FAU - Worth, L Jr
AU  - Worth L Jr
FAU - Schaaper, R M
AU  - Schaaper RM
FAU - Seio, K
AU  - Seio K
FAU - Sekine, M
AU  - Sekine M
FAU - Negishi, T
AU  - Negishi T
LA  - eng
PT  - Journal Article
PL  - England
TA  - Nucleic Acids Res Suppl
JT  - Nucleic acids research. Supplement (2001)
JID - 101169367
RN  - 0 (2'-deoxy-ribofuranosyl-(3,4-dihydro-6H,8H-pyrimido(4,5-c)(1,2)oxazin-7-one))
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Deoxyribonucleosides)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Nucleic Acid Heteroduplexes)
RN  - 0 (Oligonucleotides)
RN  - 452-06-2 (2-Aminopurine)
RN  - 57294-74-3 (N(4)-aminocytidine)
RN  - 5CSZ8459RP (Cytidine)
RN  - C3D11PV2O4 (N(4)-hydroxycytidine)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.1.3 (MutS DNA Mismatch-Binding Protein)
RN  - EC 3.6.1.3 (MutS protein, E coli)
SB  - IM
MH  - 2-Aminopurine/chemistry
MH  - Adenosine Triphosphatases/*metabolism
MH  - *Bacterial Proteins
MH  - Base Pair Mismatch
MH  - Base Sequence
MH  - Cytidine/*analogs & derivatives/chemistry
MH  - *DNA-Binding Proteins
MH  - Deoxyribonucleosides/chemistry
MH  - Escherichia coli Proteins/*metabolism
MH  - MutS DNA Mismatch-Binding Protein
MH  - Nucleic Acid Heteroduplexes/*chemistry/*metabolism
MH  - Oligonucleotides/*chemistry/*metabolism
EDAT- 2003/07/03 05:00
MHDA- 2003/08/16 05:00
CRDT- 2003/07/03 05:00
PHST- 2003/07/03 05:00 [pubmed]
PHST- 2003/08/16 05:00 [medline]
PHST- 2003/07/03 05:00 [entrez]
AID - 10.1093/nass/1.1.221 [doi]
PST - ppublish
SO  - Nucleic Acids Res Suppl. 2001;(1):221-2. doi: 10.1093/nass/1.1.221.