PMID- 12837928
OWN - NLM
STAT- MEDLINE
DCOM- 20040305
LR  - 20220321
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 550
IP  - Pt 1
DP  - 2003 Jul 1
TI  - Ghrelin induces fasted motor activity of the gastrointestinal tract in conscious 
      fed rats.
PG  - 227-40
AB  - Ghrelin is a newly discovered orexigenic peptide originating from the stomach. 
      However, its action in regulating the fed and fasted motor activity of the 
      digestive tract is not fully understood. In the present study, we examined the 
      effects of intracerebroventricular (I.C.V.) and intravenous (I.V.) injection of 
      ghrelin on the physiological fed and fasted motor activities in the stomach and 
      duodenum of freely moving conscious rats. I.C.V. and I.V. injection of ghrelin 
      induced fasted motor activity in the duodenum in normal fed rats, while I.V. 
      injection of ghrelin induced fasted motor activity in both the stomach and 
      duodenum in vagotomized rats. The effects of I.C.V. and I.V. injected ghrelin 
      were blocked by growth hormone secretagogue receptor (GHS-R) antagonist given by 
      the same route and also blocked by immunoneutralization of neuropeptide Y (NPY) 
      in the brain. The effects of I.V. injected ghrelin were not altered by I.C.V. 
      injection of GHS-R antagonist in vagotomized rats. Injection of GHS-R antagonist 
      blocked the fasted motor activity in both the stomach and duodenum in vagotomized 
      rats but did not affect the fasted motor activity in normal rats. Low 
      intragastric pH inhibited the effect of ghrelin. The present results indicate 
      that ghrelin is involved in regulation of fasted motor activity in the stomach 
      and duodenum. Peripheral ghrelin may induce the fasted motor activity by 
      activating the NPY neurons in the brain, probably through ghrelin receptors on 
      vagal afferent neurons. Once the brain mechanism is eliminated by truncal 
      vagotomy, ghrelin might be primarily involved in the regulation of fasted motor 
      activity through ghrelin receptors on the stomach and duodenum. The action of 
      ghrelin to induce fasted motor activity is strongly affected by intragastric pH; 
      low pH inhibits the action.
FAU - Fujino, Kazunori
AU  - Fujino K
AD  - Department of Surgery, Shiga University of Medical Science, Otsu, Japan.
FAU - Inui, Akio
AU  - Inui A
FAU - Asakawa, Akihiro
AU  - Asakawa A
FAU - Kihara, Naoki
AU  - Kihara N
FAU - Fujimura, Masaki
AU  - Fujimura M
FAU - Fujimiya, Mineko
AU  - Fujimiya M
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Ghrelin)
RN  - 0 (Neuropeptide Y)
RN  - 0 (Peptide Hormones)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Ghrelin)
SB  - IM
MH  - Animals
MH  - Autonomic Nervous System/physiology
MH  - Brain/physiology
MH  - Duodenum/drug effects/physiology
MH  - Fasting/*physiology
MH  - Gastric Mucosa/metabolism
MH  - Gastrointestinal Motility/*drug effects
MH  - Ghrelin
MH  - Hydrogen-Ion Concentration
MH  - Injections, Intravenous
MH  - Injections, Intraventricular
MH  - Male
MH  - Neural Pathways/physiology
MH  - Neuropeptide Y/antagonists & inhibitors
MH  - Peptide Hormones/administration & dosage/*pharmacology
MH  - *Postprandial Period
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, G-Protein-Coupled/antagonists & inhibitors
MH  - Receptors, Ghrelin
MH  - Stomach/drug effects
MH  - Sympathectomy
MH  - Vagotomy
PMC - PMC2343006
EDAT- 2003/07/03 05:00
MHDA- 2004/03/06 05:00
PMCR- 2004/07/01
CRDT- 2003/07/03 05:00
PHST- 2003/07/03 05:00 [pubmed]
PHST- 2004/03/06 05:00 [medline]
PHST- 2003/07/03 05:00 [entrez]
PHST- 2004/07/01 00:00 [pmc-release]
AID - 550/1/227 [pii]
AID - 10.1113/jphysiol.2003.040600 [doi]
PST - ppublish
SO  - J Physiol. 2003 Jul 1;550(Pt 1):227-40. doi: 10.1113/jphysiol.2003.040600.