PMID- 12838665
OWN - NLM
STAT- MEDLINE
DCOM- 20030721
LR  - 20201208
IS  - 0374-9096 (Print)
IS  - 0374-9096 (Linking)
VI  - 36
IP  - 3-4
DP  - 2002 Jul-Oct
TI  - [Molecular epidemiologic surveillance and antifungal agent sensitivity of Candida 
      albicans isolated from anesthesia intensive care units].
PG  - 309-16
AB  - Patients in intensive care units (ICU) are at risk of nosocomial infections. The 
      incidence of nosocomial fungal infections has increased in parallel with the 
      increase of nosocomial infections. Candida albicans is the most frequent 
      pathogenic species among the fungi. The aim of this study was to make an 
      epidemiological surveillance of C. albicans urine isolates which were isolated 
      from patients who were hospitalized in ICU between June 2000 and October 2001 by 
      antifungal susceptibility testing and Randomly Amplified Polymorphic DNA (RAPD) 
      analysis. For this purpose, 38 C. albicans which were isolated from 29 patients 
      were investigated for amphotericin B and fluconazole susceptibility with the 
      microdilution method. The range of minimal inhibitory concentration (MIC) of 
      amphotericin B was between 0.25-1 microgram/ml and MIC50 value was 0.5 
      microgram/ml and none of the isolates had high (MIC > 1 microgram/ml) MIC values. 
      The MIC values for fluconazole varied between 0.25-16 micrograms/ml and MIC50 
      value was 1 microgram/ml. While none of the isolates was resistant to 
      fluconazole, two isolates were detected as dose dependent susceptible. RAPD 
      analysis was performed with two different primers in order to investigate clonal 
      relationship, and 22 patterns were detected with one of the primers and 24 
      patterns were detected with the other. In conclusion, it is thought that the 
      origin of the C. albicans urine isolates were mostly endogenous but exogenous 
      spread might also be considered as isolates that were clonally related were 
      isolated from different patients at the same time interval.
FAU - Gulay, Zeynep
AU  - Gulay Z
AD  - Dokuz Eylul Universitesi Tip Fakultesi Hastanesi, Mikrobiyoloji ve Klinik 
      Mikrobiyoloji Anabilim Dali, Izmir.
FAU - Ergon, Cem
AU  - Ergon C
FAU - Ozkutuk, Aydan
AU  - Ozkutuk A
FAU - Yucesoy, Mine
AU  - Yucesoy M
FAU - Bicmen, Meral
AU  - Bicmen M
LA  - tur
PT  - Journal Article
TT  - Anestezi yogun bakim unitesi hastalarindan izole edilen Candida albicans 
      suslarinin antifungal ajanlara duyarliligi ve molekuler epidemiyolojik izlemi.
PL  - Turkey
TA  - Mikrobiyol Bul
JT  - Mikrobiyoloji bulteni
JID - 7503830
RN  - 0 (Antifungal Agents)
RN  - 0 (DNA, Fungal)
RN  - 7XU7A7DROE (Amphotericin B)
RN  - 8VZV102JFY (Fluconazole)
SB  - IM
MH  - Amphotericin B/pharmacology
MH  - Antifungal Agents/*pharmacology
MH  - Candida albicans/classification/drug effects/genetics/*isolation & purification
MH  - Candidiasis/epidemiology/*microbiology/urine
MH  - Cross Infection/epidemiology/*microbiology/urine
MH  - DNA, Fungal/*chemistry/isolation & purification
MH  - Dose-Response Relationship, Drug
MH  - Fluconazole/pharmacology
MH  - Humans
MH  - Incidence
MH  - Intensive Care Units
MH  - Microbial Sensitivity Tests
MH  - Random Amplified Polymorphic DNA Technique
MH  - Turkey/epidemiology
MH  - Urine/microbiology
EDAT- 2003/07/04 05:00
MHDA- 2003/07/23 05:00
CRDT- 2003/07/04 05:00
PHST- 2003/07/04 05:00 [pubmed]
PHST- 2003/07/23 05:00 [medline]
PHST- 2003/07/04 05:00 [entrez]
PST - ppublish
SO  - Mikrobiyol Bul. 2002 Jul-Oct;36(3-4):309-16.