PMID- 12840897
OWN - NLM
STAT- MEDLINE
DCOM- 20031027
LR  - 20240202
IS  - 0257-716X (Print)
IS  - 0257-716X (Linking)
VI  - 19
IP  - 3
DP  - 1999
TI  - Effect of IL-1 beta and TNF-alpha on the expression of monocyte chemotactic 
      protein-1 in endometriotic cells.
PG  - 212-4
AB  - To investigate the clinical significance of monocyte chemotactic protein-1 
      (MCP-1) produced by endometriotic tissues, the endometriotic tissues were taken 
      from 15 patients with endometriosis. MCP-1 mRNA and MCP-1 protein were determined 
      by dot blot analysis and enzyme linked immunosorbent assay (ELISA) in 
      endometriotic cells cultured with or without interleukin-1 beta (IL-1 beta, 2 
      micrograms/L), tumor necrosis factor-alpha (TNF-alpha, 20 g/L). After exposure to 
      IL-1 beta or TNF-alpha, the expression of MCP-1 mRNA in the endometriotic cells 
      (8.635 +/- 0.826, 7.031 +/- 0.970, respectively) were significantly higher than 
      that in the control group (4.482 +/- 0.435, P < 0.05); The expression of MCP-1 
      protein in IL-1 beta and TNF-alpha group was 4.52 +/- 0.09 micrograms/L, 2.87 +/- 
      0.27 micrograms/L, respectively, which were significantly higher than 1.74 +/- 
      0.16 micrograms/L in control (P < 0.01). The results suggested that IL-1 beta and 
      TNF-alpha could up-regulate the expression of MCP-1 in endometriotic cells, which 
      might be related to the development of endometriosis.
FAU - Gao, Y
AU  - Gao Y
AD  - Department of Obstetrics and Gynecology, Xiehe Hospital, Tongji Medical 
      University, Wuhan 430022.
FAU - Luo, L
AU  - Luo L
FAU - He, F
AU  - He F
LA  - eng
PT  - Journal Article
PL  - China
TA  - J Tongji Med Univ
JT  - Journal of Tongji Medical University = Tong ji yi ke da xue xue bao
JID - 8605495
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Culture Media, Conditioned
MH  - Endometriosis/*metabolism/pathology
MH  - Endometrium/*metabolism/pathology
MH  - Female
MH  - Humans
MH  - Interleukin-1/*pharmacology
MH  - Peritoneal Diseases/metabolism/pathology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2003/07/05 05:00
MHDA- 2003/10/28 05:00
CRDT- 2003/07/05 05:00
PHST- 2003/07/05 05:00 [pubmed]
PHST- 2003/10/28 05:00 [medline]
PHST- 2003/07/05 05:00 [entrez]
AID - 10.1007/BF02887737 [doi]
PST - ppublish
SO  - J Tongji Med Univ. 1999;19(3):212-4. doi: 10.1007/BF02887737.