PMID- 12841651
OWN - NLM
STAT- MEDLINE
DCOM- 20040415
LR  - 20190818
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 247
IP  - 1-2
DP  - 2003 May
TI  - Genistein protects primary cortical neurons from iron-induced lipid peroxidation.
PG  - 219-22
AB  - Neuroprotective role of isoflavones in particular genistein might be resulted 
      from their antioxidant activities in addition to their estrogenic actions. In the 
      present study, we investigated effect of genistein on iron-induced free radical 
      reaction in cultured cortical neurons. Thiobarbituric-acid-reactive species 
      (TBARS) and superoxide dismutase (SOD) were measured after incubation of the 
      cells with different concentrations of genistein in the absence or presence of 
      iron (12.5 microM) for 24 h. Genistein at 100 microM significantly reduced the 
      iron-induced TBARS, implying that genistein has an inhibitory role on 
      iron-induced lipid peroxidation. Also, genistein (100 microM) led to a relatively 
      higher SOD level than that in iron treatment although no significant difference 
      was found. The findings imply that the antioxidative effect of genistein is 
      partly associated with its neuroprotective function.
FAU - Ho, Kwok Ping
AU  - Ho KP
AD  - Laboratory of Brain Iron Metabolism, Department of Applied Biology and Chemical 
      Technology, The Hong Kong Polytechnic University, Kowloon, Hong Kong, Peoples 
      Republic of China. bczmqian@polyu.edu.hk
FAU - Li, Lin
AU  - Li L
FAU - Zhao, Li
AU  - Zhao L
FAU - Qian, Zhong Ming
AU  - Qian ZM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Antioxidants)
RN  - 0 (Free Radicals)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - DH2M523P0H (Genistein)
RN  - E1UOL152H7 (Iron)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology/*drug effects/metabolism
MH  - Free Radicals/metabolism
MH  - Genistein/*pharmacology
MH  - Iron/*pharmacology
MH  - Lipid Peroxidation/*drug effects
MH  - Neurons/*drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Superoxide Dismutase/drug effects/metabolism
MH  - Thiobarbituric Acid Reactive Substances/metabolism
EDAT- 2003/07/05 05:00
MHDA- 2004/04/16 05:00
CRDT- 2003/07/05 05:00
PHST- 2003/07/05 05:00 [pubmed]
PHST- 2004/04/16 05:00 [medline]
PHST- 2003/07/05 05:00 [entrez]
AID - 10.1023/a:1024142004575 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2003 May;247(1-2):219-22. doi: 10.1023/a:1024142004575.