PMID- 12842610
OWN - NLM
STAT- MEDLINE
DCOM- 20031027
LR  - 20190901
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 94
IP  - 1-2
DP  - 2003 Jul 15
TI  - Analysis of constitutive cytokine expression by pigs infected in-utero with 
      porcine reproductive and respiratory syndrome virus.
PG  - 35-45
AB  - To investigate cytokine alterations in pigs infected in-utero with porcine 
      reproductive and respiratory syndrome virus (PRRSV), constitutive mRNA expression 
      by peripheral blood mononuclear cells (PBMCs) was measured. PBMC from in-utero 
      PRRSV-infected pigs displayed significantly increased IL-6, IL-10, and IFN-gamma 
      mRNA expression at 0 and 14 days of age compared with age-matched control pigs. 
      There were no significant differences in IL-2, IL-4, and IL-12 mRNA expression 
      between in-utero PRRSV-infected and control pigs. However, the IL-10/IL-12 ratio 
      was significantly increased in in-utero PRRSV-infected pigs at 0 and 14 days of 
      age, suggesting the imbalance of IL-10 and IL-12 mRNA production. The abnormal 
      mRNA expression of cytokines in in-utero PRRSV-infected pigs occurred 
      concurrently with a significant decrease in the CD4(+)/CD8(+) T-cell ratio in 
      peripheral blood. PRRSV was not isolated from the sera of pigs at 9 weeks of age 
      that had been viremic at 0 and 14 days old. Delayed type hypersensitivity (DTH) 
      responses to Tuberculin and analysis of cytokine mRNA expression by PBMC showed 
      that cell-mediated immune response and cytokine message profiles in pigs infected 
      in-utero with PRRSV had returned to levels similar to those of control pigs by 9 
      weeks of age. We conclude that in-utero infection with PRRSV results in 
      significant alteration of cytokine mRNA expression that may cause transient 
      immunomodulation. However, at 10 weeks of age the pigs' immune responses seemed 
      to recover. This may help to understand the immunopathogenesis of in-utero PRRSV 
      infection and the increased susceptibility to secondary bacterial pathogens in 
      neonatal piglets.
FAU - Feng, W-H
AU  - Feng WH
AD  - Department of Farm Animal Health and Resource Management, College of Veterinary 
      Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 
      27606, USA.
FAU - Tompkins, M B
AU  - Tompkins MB
FAU - Xu, J-S
AU  - Xu JS
FAU - Zhang, H-X
AU  - Zhang HX
FAU - McCaw, M B
AU  - McCaw MB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Cytokines/genetics/*immunology
MH  - Gene Expression Regulation
MH  - Hypersensitivity, Delayed/immunology
MH  - Leukocytes, Mononuclear/immunology/metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/*congenital/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/immunology/isolation & 
      purification
MH  - RNA, Messenger/analysis
MH  - Swine/*immunology/*virology
EDAT- 2003/07/05 05:00
MHDA- 2003/10/28 05:00
CRDT- 2003/07/05 05:00
PHST- 2003/07/05 05:00 [pubmed]
PHST- 2003/10/28 05:00 [medline]
PHST- 2003/07/05 05:00 [entrez]
AID - S016524270300059X [pii]
AID - 10.1016/s0165-2427(03)00059-x [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2003 Jul 15;94(1-2):35-45. doi: 
      10.1016/s0165-2427(03)00059-x.