PMID- 12843269
OWN - NLM
STAT- MEDLINE
DCOM- 20030814
LR  - 20200225
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 23
IP  - 13
DP  - 2003 Jul 2
TI  - Brain-derived neurotrophic factor mediates activity-dependent dendritic growth in 
      nonpyramidal neocortical interneurons in developing organotypic cultures.
PG  - 5662-73
AB  - Brain-derived neurotrophic factor (BDNF) promotes postnatal maturation of 
      GABAergic inhibition in the cerebral and cerebellar cortices, and its expression 
      and release are enhanced by neuronal activity, suggesting that it acts in a 
      feedback manner to maintain a balance between excitation and inhibition during 
      development. BDNF promotes differentiation of cerebellar, hippocampal, and 
      neostriatal inhibitory neurons, but its effects on the dendritic development of 
      neocortical inhibitory interneurons remain unknown. Here, we show that BDNF 
      mediates depolarization-induced dendritic growth and branching in neocortical 
      interneurons. To visualize inhibitory interneurons, we biolistically transfected 
      organotypic cortical slice cultures from neonatal mice with green fluorescent 
      protein (GFP) driven by the glutamic acid decarboxylase (GAD)67 promoter. Nearly 
      all GAD67-GFP-expressing neurons were nonpyramidal, many contained GABA, and some 
      expressed markers of neurochemically defined GABAergic subtypes, indicating that 
      GAD67-GFP-expressing neurons were GABAergic. We traced dendritic trees from 
      confocal images of the same GAD67-GFP-expressing neurons before and after a 5 d 
      growth period, and quantified the change in total dendritic length (TDL) and 
      total dendritic branch points (TDBPs) for each neuron. GAD67-GFP-expressing 
      neurons growing in control medium exhibited a 20% increase in TDL, but in 200 
      ng/ml BDNF or 10 mm KCl, this increase nearly doubled and was accompanied by a 
      significant increase in TDBPs. Blocking action potentials with TTX did not 
      prevent the BDNF-induced growth, but antibodies against BDNF blocked the 
      growth-promoting effect of KCl. We conclude that BDNF, released by neocortical 
      pyramidal neurons in response to depolarization, enhances dendritic growth and 
      branching in nearby inhibitory interneurons.
FAU - Jin, Xiaoming
AU  - Jin X
AD  - Department of Neurobiology and Anatomy, West Virginia University, Morgantown, 
      West Virginia 26506-9128, USA.
FAU - Hu, Hang
AU  - Hu H
FAU - Mathers, Peter H
AU  - Mathers PH
FAU - Agmon, Ariel
AU  - Agmon A
LA  - eng
GR  - R01 EY012152/EY/NEI NIH HHS/United States
GR  - R29 EY012152/EY/NEI NIH HHS/United States
GR  - EY12152/EY/NEI NIH HHS/United States
GR  - HD33463/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Isoenzymes)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 145172-44-7 (neurotrophin 5)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - EC 4.1.1.15 (Glutamate Decarboxylase)
RN  - EC 4.1.1.15 (glutamate decarboxylase 1)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Biolistics
MH  - Brain-Derived Neurotrophic Factor/antagonists & 
      inhibitors/pharmacology/*physiology
MH  - Cell Division/drug effects/physiology
MH  - Cells, Cultured
MH  - Dendrites/drug effects/*physiology
MH  - Glutamate Decarboxylase/genetics
MH  - Green Fluorescent Proteins
MH  - Immunohistochemistry
MH  - In Vitro Techniques
MH  - Interneurons/cytology/drug effects/*physiology
MH  - Isoenzymes/genetics
MH  - Luminescent Proteins/biosynthesis/genetics
MH  - Mice
MH  - Neocortex/cytology/*growth & development/*physiology
MH  - Nerve Growth Factors/pharmacology
MH  - Neural Inhibition/physiology
MH  - Promoter Regions, Genetic
MH  - Time Factors
MH  - gamma-Aminobutyric Acid/metabolism
PMC - PMC6741232
EDAT- 2003/07/05 05:00
MHDA- 2003/08/15 05:00
PMCR- 2004/01/02
CRDT- 2003/07/05 05:00
PHST- 2003/07/05 05:00 [pubmed]
PHST- 2003/08/15 05:00 [medline]
PHST- 2003/07/05 05:00 [entrez]
PHST- 2004/01/02 00:00 [pmc-release]
AID - 23/13/5662 [pii]
AID - 10.1523/JNEUROSCI.23-13-05662.2003 [doi]
PST - ppublish
SO  - J Neurosci. 2003 Jul 2;23(13):5662-73. doi: 10.1523/JNEUROSCI.23-13-05662.2003.