PMID- 12843289 OWN - NLM STAT- MEDLINE DCOM- 20030814 LR - 20200225 IS - 1529-2401 (Electronic) IS - 0270-6474 (Print) IS - 0270-6474 (Linking) VI - 23 IP - 13 DP - 2003 Jul 2 TI - The trophic role of oligodendrocytes in the basal forebrain. PG - 5846-53 AB - Traditionally, the primary function of oligodendrocytes (OLGs) in the CNS has been considered to be myelination. Here, we investigated whether OLGs may play a trophic role, particularly during development. Neurotrophin expression was assessed in postnatal day 7 basal forebrain (BF) OLGs, using in situ hybridization and detection of myelin basic protein. Nerve growth factor, brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) mRNAs were revealed in OLGs in vivo and in culture. To determine whether OLGs support nearby neurons, we examined the influence of OLGs on BF cholinergic neurons. Neuronal function was enhanced by cocultured OLGs and OLG conditioned medium. Moreover, trophic effects of OLG conditioned medium were partially blocked by K252a, a trk tyrosine kinase inhibitor, and by neutralizing anti-BDNF or anti-NT-3 antisera, indicating that neurotrophins may mediate these effects, perhaps in concert with other signals. Our studies support a novel role for OLGs in providing local trophic support for neurons in the CNS. FAU - Dai, Xudong AU - Dai X AD - Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA. FAU - Lercher, Lauren D AU - Lercher LD FAU - Clinton, Patricia M AU - Clinton PM FAU - Du, Yangzhou AU - Du Y FAU - Livingston, Denise L AU - Livingston DL FAU - Vieira, Cristina AU - Vieira C FAU - Yang, Lu AU - Yang L FAU - Shen, Michael M AU - Shen MM FAU - Dreyfus, Cheryl F AU - Dreyfus CF LA - eng GR - HL60212/HL/NHLBI NIH HHS/United States GR - R01 HD038766/HD/NICHD NIH HHS/United States GR - HD38766/HD/NICHD NIH HHS/United States GR - R56 NS036647/NS/NINDS NIH HHS/United States GR - R01 NS036647/NS/NINDS NIH HHS/United States GR - NS36647/NS/NINDS NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - J Neurosci JT - The Journal of neuroscience : the official journal of the Society for Neuroscience JID - 8102140 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Carbazoles) RN - 0 (Culture Media, Conditioned) RN - 0 (Enzyme Inhibitors) RN - 0 (Immune Sera) RN - 0 (Indole Alkaloids) RN - 0 (Myelin Basic Protein) RN - 0 (Neurotrophin 3) RN - 0 (RNA, Messenger) RN - 9061-61-4 (Nerve Growth Factor) RN - 97161-97-2 (staurosporine aglycone) SB - IM MH - Animals MH - Astrocytes/cytology/physiology MH - Brain-Derived Neurotrophic Factor/antagonists & inhibitors/biosynthesis/genetics MH - Carbazoles/pharmacology MH - Cell Division/drug effects/physiology MH - Cell Lineage MH - Cells, Cultured MH - Cholinergic Fibers/drug effects/physiology MH - Coculture Techniques MH - Culture Media, Conditioned/pharmacology MH - Enzyme Inhibitors/pharmacology MH - Immune Sera/pharmacology MH - Indole Alkaloids MH - Microglia/cytology/physiology MH - Myelin Basic Protein/biosynthesis MH - Nerve Growth Factor/antagonists & inhibitors/biosynthesis/genetics MH - Neurons/cytology/drug effects/physiology MH - Neurotrophin 3/antagonists & inhibitors/biosynthesis/genetics MH - Oligodendroglia/cytology/metabolism/*physiology MH - Prosencephalon/cytology/*growth & development/*physiology MH - RNA, Messenger/biosynthesis MH - Rats MH - Rats, Sprague-Dawley PMC - PMC6741226 EDAT- 2003/07/05 05:00 MHDA- 2003/08/15 05:00 PMCR- 2004/01/02 CRDT- 2003/07/05 05:00 PHST- 2003/07/05 05:00 [pubmed] PHST- 2003/08/15 05:00 [medline] PHST- 2003/07/05 05:00 [entrez] PHST- 2004/01/02 00:00 [pmc-release] AID - 23/13/5846 [pii] AID - 10.1523/JNEUROSCI.23-13-05846.2003 [doi] PST - ppublish SO - J Neurosci. 2003 Jul 2;23(13):5846-53. doi: 10.1523/JNEUROSCI.23-13-05846.2003.