PMID- 12843450
OWN - NLM
STAT- MEDLINE
DCOM- 20091208
LR  - 20030704
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 181
DP  - 2001
TI  - Flow cytometry and FISH to investigate allele-specific replication timing and 
      homologous association of imprinted chromosomes.
PG  - 181-92
AB  - Chromosome replication banding studies show that homologous regions on a pair of 
      autosomes generally replicate at the same time in S phase (1). Izumikawa et al. 
      first observed that this was not the case for the imprinted chromosomal region 
      15q11-q13 (2). This observation has been confirmed in other replication banding 
      studies (3) as well by the fluorescence in situ hybridization (FISH) replication 
      assay (4-9). The latter technique has also been used to observe DNA replication 
      asynchrony in association with allelic inactivation of genes such as those 
      encoding olfactory receptors and the cytokine, interleukin 2 (10,11). The latter 
      genes are not imprinted but display random silencing of an allele in individual 
      cells. In imprinted regions, DNA replication was generally observed to occur 
      earlier on the paternal homologue (5,6,9,12,13). The patterns of allele-specific 
      replication in the cells of Prader- Willi (PWS) and Angelman syndrome (AS) 
      patients, however, have generally been synchronous (5,6,14). Furthermore, an 
      investigation of the kinetics of allele-specific replication timing in the 
      GABRB3/A5 cluster on 15q11-13 revealed that cells from PWS and AS have lost the 
      strict replication timing observed on the parental chromosomes of normal cells 
      (12). These results suggested the requirement of a biparental contribution for 
      the regulation of replication asynchrony and lead to the hypothesis that allelic 
      cross-talk, perhaps via pairing of homologous chromosomes, might play a role in 
      the imprinting process.
FAU - LaSalle, J
AU  - LaSalle J
AD  - Medical Microbiology and Immunology, School of Medicine, University of 
      California, Davis, CA, USA.
FAU - Lalande, M
AU  - Lalande M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - *Alleles
MH  - Animals
MH  - Cells, Cultured
MH  - DNA Replication/*genetics
MH  - Flow Cytometry/*methods
MH  - Genomic Imprinting/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
EDAT- 2003/07/05 05:00
MHDA- 2009/12/16 06:00
CRDT- 2003/07/05 05:00
PHST- 2003/07/05 05:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
PHST- 2003/07/05 05:00 [entrez]
AID - 1-59259-211-2-181 [pii]
AID - 10.1385/1-59259-211-2:181 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2001;181:181-92. doi: 10.1385/1-59259-211-2:181.