PMID- 12843613
OWN - NLM
STAT- MEDLINE
DCOM- 20040304
LR  - 20211203
IS  - 0918-6158 (Print)
IS  - 0918-6158 (Linking)
VI  - 26
IP  - 7
DP  - 2003 Jul
TI  - Activation signal of nuclear factor-kappa B in response to endoplasmic reticulum 
      stress is transduced via IRE1 and tumor necrosis factor receptor-associated 
      factor 2.
PG  - 931-5
AB  - Conditions that perturb the function of the endoplasmic reticulum (ER) lead to an 
      accumulation of proteins and subsequent induction of several responses, such as 
      an increased expression of ER-resident chaperones involved in protein folding and 
      activation of c-jun N-terminal kinase (JNK). These responses are mediated by a 
      transmembrane kinase/ribonuclease, IRE1, which transduces the signal from the ER 
      lumen to the cytosol. Although nuclear transcription factor-kappaB (NF-kappaB) is 
      also activated by ER stress, whether this response depends on IRE1 is unknown. In 
      this study, we show that IRE1 is involved in the activation of NF-kappaB induced 
      by ER stress. NF-kappaB was activated by ER stress-inducing agents, thapsigargin 
      and tunicamycin. The activation was inhibited by a dominant-negative IRE1. In 
      addition, a dominant-negative TRAF2 also suppressed the activation of NF-kappaB 
      by ER stress. These results suggest that ER stress-induced NF-kappaB activation 
      is also mediated by the IRE1-TRAF2 pathway, as well as JNK activation.
FAU - Kaneko, Masayuki
AU  - Kaneko M
AD  - Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Hokkaido 
      University, Sapporo, Japan.
FAU - Niinuma, Yoshifumi
AU  - Niinuma Y
FAU - Nomura, Yasuyuki
AU  - Nomura Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Membrane Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Proteins)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 2.7.1.- (ERN2 protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 3.1.- (Endoribonucleases)
SB  - IM
MH  - Cell Line
MH  - Endoplasmic Reticulum/drug effects/genetics/*metabolism
MH  - Endoribonucleases
MH  - Humans
MH  - Membrane Proteins/genetics/*metabolism
MH  - NF-kappa B/genetics/*metabolism
MH  - Protein Serine-Threonine Kinases/genetics/*metabolism
MH  - Proteins/genetics/*metabolism
MH  - Signal Transduction/drug effects/*physiology
MH  - TNF Receptor-Associated Factor 2
MH  - Thapsigargin/toxicity
EDAT- 2003/07/05 05:00
MHDA- 2004/03/05 05:00
CRDT- 2003/07/05 05:00
PHST- 2003/07/05 05:00 [pubmed]
PHST- 2004/03/05 05:00 [medline]
PHST- 2003/07/05 05:00 [entrez]
AID - 10.1248/bpb.26.931 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2003 Jul;26(7):931-5. doi: 10.1248/bpb.26.931.