PMID- 12845499
OWN - NLM
STAT- MEDLINE
DCOM- 20031104
LR  - 20181113
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 55
IP  - 5
DP  - 2003 Aug
TI  - A single nucleotide polymorphism in the Emp3 gene defines the H4 minor 
      histocompatibility antigen.
PG  - 284-95
AB  - Minor histocompatibility antigens (minor H antigen) elicit strong T-cell-mediated 
      responses during both graft rejection and graft versus leukemia (GvL) among 
      MHC-matched individuals (where MHC is major histocompatibility complex). 
      Employing expression-cloning methodology, we have identified a cDNA clone, MI-35, 
      encoding the immunodominant H4b minor H antigen within the classical mouse H4 
      complex. The minimal antigenic epitope derived from H4b presented on Kb class I 
      MHC is SGIVYIHL (SYL8) and the polymorphism is due to C-->T nucleotide 
      modification in p3 resulting in the change of threonine (ACT) to isoleucine 
      (ATT). The results presented here demonstrate that amino acid variation in the 
      allelic epitopes results in the low abundance of H4a peptide. The differential 
      peptide copy number resulted in an immunodominant cytotoxic T cells (CTL) 
      response directed against H4b while the anti-B6 response directed against H4a was 
      easily dominated. These results provide a molecular mechanism for the H4 minor H 
      antigen and suggest a novel mechanism by which alloantigenic disparity caused by 
      conservative amino acid changes can be augmented by posttranslational antigen 
      processing events.
FAU - Luedtke, Brianna
AU  - Luedtke B
AD  - Laboratory of Molecular Immunology, Blood Research Institute, Blood Center of 
      South-eastern Wisconsin, Milwaukee, WI 53226-2178, USA.
FAU - Pooler, Laura M
AU  - Pooler LM
FAU - Choi, Eun Young
AU  - Choi EY
FAU - Tranchita, Angela M
AU  - Tranchita AM
FAU - Reinbold, Corbett J A
AU  - Reinbold CJ
FAU - Brown, Aaron C
AU  - Brown AC
FAU - Shaffer, Daniel J
AU  - Shaffer DJ
FAU - Roopenian, Derry C
AU  - Roopenian DC
FAU - Malarkannan, Subramaniam
AU  - Malarkannan S
LA  - eng
GR  - R01 AI28802/AI/NIAID NIH HHS/United States
GR  - R01 HL65479/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030704
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (DNA, Complementary)
RN  - 0 (EMP3 protein, human)
RN  - 0 (Emp3 protein, mouse)
RN  - 0 (Emp3 protein, rat)
RN  - 0 (Epitopes)
RN  - 0 (Isoantigens)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Minor Histocompatibility Antigens)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - DNA, Complementary
MH  - Epitopes
MH  - Isoantigens/genetics
MH  - Membrane Glycoproteins/*genetics
MH  - Mice
MH  - Minor Histocompatibility Antigens/*genetics
MH  - Molecular Sequence Data
MH  - *Polymorphism, Single Nucleotide
EDAT- 2003/07/08 05:00
MHDA- 2003/11/05 05:00
CRDT- 2003/07/08 05:00
PHST- 2003/03/03 00:00 [received]
PHST- 2003/05/12 00:00 [revised]
PHST- 2003/07/08 05:00 [pubmed]
PHST- 2003/11/05 05:00 [medline]
PHST- 2003/07/08 05:00 [entrez]
AID - 10.1007/s00251-003-0581-x [doi]
PST - ppublish
SO  - Immunogenetics. 2003 Aug;55(5):284-95. doi: 10.1007/s00251-003-0581-x. Epub 2003 
      Jul 4.