PMID- 12845634
OWN - NLM
STAT- MEDLINE
DCOM- 20030916
LR  - 20220408
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 200
IP  - 3
DP  - 2003 Jul
TI  - Cyclin E overexpression and amplification in human tumours.
PG  - 375-82
AB  - Cyclin E amplification and overexpression have recently been described in several 
      tumour types. However, many tumour entities have never been examined for cyclin E 
      alterations. Numerous and time-consuming experiments were previously required to 
      determine the significance of potential oncogenes across different tumour types. 
      To overcome this problem, tissue microarrays (TMAs) consisting of 3670 primary 
      tumours from 128 different tumour types, 709 metastases, and 354 normal tissues 
      were generated. Cyclin E alterations were then analysed by fluorescence in situ 
      hybridization (FISH) and immunohistochemistry (IHC). Cyclin E gene amplification 
      was observed in 15 different tumour types and subtypes, ie rhabdomyosarcoma, 
      urinary bladder cancer (three subtypes), ovarian cancer (two subtypes), malignant 
      fibrous histiocytoma, adenocarcinoma of the small intestine, medullary breast 
      cancer, gall bladder adenocarcinoma, phaeochromocytoma, gastric adenocarcinoma, 
      squamous cell carcinoma of the uterine cervix, colonic adenocarcinoma, and 
      endometrial carcinoma. Cyclin E protein accumulation was found in 48 different 
      tumour types. The use of TMA technology has enabled us to expand considerably our 
      knowledge of cyclin E alterations in human tumours. The occurrence of 
      amplification and overexpression in many different tumour types suggests that 
      cyclin E plays an important role in tumour biology.
CI  - Copyright 2003 John Wiley & Sons, Ltd.
FAU - Schraml, Peter
AU  - Schraml P
AD  - Institute of Pathology, University Hospital, University of Basel, 4031 Basel, 
      Switzerland. Peter.Schraml@unibas.ch
FAU - Bucher, Christoph
AU  - Bucher C
FAU - Bissig, Heidi
AU  - Bissig H
FAU - Nocito, Antonio
AU  - Nocito A
FAU - Haas, Philippe
AU  - Haas P
FAU - Wilber, Kim
AU  - Wilber K
FAU - Seelig, Steven
AU  - Seelig S
FAU - Kononen, Juha
AU  - Kononen J
FAU - Mihatsch, Michael J
AU  - Mihatsch MJ
FAU - Dirnhofer, Stefan
AU  - Dirnhofer S
FAU - Sauter, Guido
AU  - Sauter G
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (Cyclin E)
SB  - IM
MH  - Cyclin E/analysis/*genetics
MH  - Gene Amplification/*genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Immunoenzyme Techniques/methods
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Neoplasm Metastasis
MH  - Neoplasms/*genetics/metabolism
MH  - Oligonucleotide Array Sequence Analysis/methods
EDAT- 2003/07/08 05:00
MHDA- 2003/09/17 05:00
CRDT- 2003/07/08 05:00
PHST- 2003/07/08 05:00 [pubmed]
PHST- 2003/09/17 05:00 [medline]
PHST- 2003/07/08 05:00 [entrez]
AID - 10.1002/path.1356 [doi]
PST - ppublish
SO  - J Pathol. 2003 Jul;200(3):375-82. doi: 10.1002/path.1356.