PMID- 12846558
OWN - NLM
STAT- MEDLINE
DCOM- 20031007
LR  - 20191025
IS  - 0914-9465 (Print)
IS  - 0914-9465 (Linking)
VI  - 66
IP  - 2
DP  - 2003 May
TI  - The involvement of brain-derived neurotrophic factor (BDNF) in the regeneration 
      of periodontal Ruffini endings following transection of the inferior alveolar 
      nerve.
PG  - 183-94
AB  - The present study employed immunohistochemistry for protein gene product 9.5 (PGP 
      9.5) to examine the regeneration process of Ruffini endings, the primary 
      mechanoreceptor in the periodontal ligament, in heterozygous mice with targeted 
      disruption of the brain-derived neurotrophic factor (BDNF) gene and their 
      littermates, following transection of the inferior alveolar nerve. When 
      immunostained for PGP 9.5, periodontal Ruffini endings appeared densely 
      distributed in the periodontal ligament of the heterozygous mice, but the density 
      of the positively stained nerve fibers in the ligament was 20% lower than that in 
      the control littermates. At 3 days after surgery, the PGP 9.5-positive neural 
      elements had disappeared; they began to appear in the periodontal ligament of 
      both animals at 7 days. However, the recovery pattern of the PGP 9.5-positive 
      nerves differed between heterozygous and wild type mice, typical periodontal 
      Ruffini endings morphologically identical to those in the control group appeared 
      in the wild-type mice at 7 days, whereas such Ruffini endings were detectable in 
      the heterozygous mice at 28 days, though much smaller in number. On day 28, when 
      PGP 9.5-positive nerves were largely regenerated in wild type mice, their 
      distribution was much less dense in the ligament of the heterozygous mice than in 
      the non-treated heterozygous mice. The density of PGP 9.5-positive nerve fibers 
      was significantly lower in the heterozygous mice than in wild type mice at any 
      stage examined. These data showing that a reduced expression of BDNF causes 
      delayed regeneration of the periodontal Ruffini endings suggest the involvement 
      of BDNF in the regeneration process of these mechanoreceptors.
FAU - Harada, Fumiko
AU  - Harada F
AD  - Division of Oral Anatomy, Department of Oral Biological Science, Niigata 
      University Graduate School of Medical and Dental Sciences, Niigata, Japan.
FAU - Hoshino, Natalia
AU  - Hoshino N
FAU - Hanada, Kooji
AU  - Hanada K
FAU - Kawano, Yoshiro
AU  - Kawano Y
FAU - Atsumi, Yukako
AU  - Atsumi Y
FAU - Wakisaka, Satoshi
AU  - Wakisaka S
FAU - Maeda, Takeyasu
AU  - Maeda T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Arch Histol Cytol
JT  - Archives of histology and cytology
JID - 8806082
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 3.1.2.- (Thiolester Hydrolases)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Genotype
MH  - Heterozygote
MH  - Immunohistochemistry
MH  - Mandibular Nerve/*surgery
MH  - Mechanoreceptors/*physiology
MH  - Mice
MH  - Nerve Fibers/physiology
MH  - Nerve Regeneration/*physiology
MH  - Periodontal Ligament/*physiology
MH  - Thiolester Hydrolases/metabolism
MH  - Time Factors
MH  - Tissue Distribution
MH  - Ubiquitin Thiolesterase
EDAT- 2003/07/09 05:00
MHDA- 2003/10/08 05:00
CRDT- 2003/07/09 05:00
PHST- 2003/07/09 05:00 [pubmed]
PHST- 2003/10/08 05:00 [medline]
PHST- 2003/07/09 05:00 [entrez]
AID - 10.1679/aohc.66.183 [doi]
PST - ppublish
SO  - Arch Histol Cytol. 2003 May;66(2):183-94. doi: 10.1679/aohc.66.183.