PMID- 12847317
OWN - NLM
STAT- MEDLINE
DCOM- 20030820
LR  - 20190916
IS  - 1077-4114 (Print)
IS  - 1077-4114 (Linking)
VI  - 25
IP  - 7
DP  - 2003 Jul
TI  - Preponderant mitotic activity of nonleukemic cells plays an important role in 
      failures to detect abnormal clone in childhood acute lymphoblastic leukemia.
PG  - 520-5
AB  - At diagnosis, clonal chromosomal abnormalities are found in the bone marrow 
      blasts in more than two thirds of children with acute lymphoblastic leukemia 
      (ALL). Practically, however, failure to detect these abnormalities is frequent 
      and usually attributed to poor marrow sampling, inadequate metaphases, and/or a 
      preponderant mitotic activity among nonleukemic cells. The authors applied 
      fluorescence in situ hybridization (FISH) techniques to re-examine 30 cases of 
      karyotypically "normal" childhood ALL to explore the role of preponderant mitotic 
      activities of nonleukemic cells in failures to detect clonal abnormalities. The 
      FISH test were performed using TEL/AML1 fusion gene probe and the centromere 
      probes for chromosome 8 and 10 to detect the t(12;21) translocation and/or 
      hyperdiploidy. Half of the karyotypically "normal" ALL cases examined have been 
      found to have abnormal clones with t(12;21) rearrangement and/or hyperdiploidy by 
      this specially designed FISH assay. Contrary to expectation, the authors found a 
      higher incidence (52%) of clonal abnormalities in cases where over 20 metaphases 
      had been examined than in cases (44%) where fewer than 20 metaphases had been 
      analyzed. These findings suggest that a preponderant mitotic activity of 
      nonleukemic cells plays an important role in failures to detect an abnormal clone 
      by conventional cytogenetic studies. Therefore, karyotypically "normal" childhood 
      ALL patients should undergo FISH studies to rule out the presence of t(12;21) 
      and/or hyperdiploid clone.
FAU - Wu, Shi Qi
AU  - Wu SQ
AD  - Department of Pediatrics, Children's Hospital Los Angeles, Keck School of 
      Medicine, University of Southern California, 4650 Sunset Blvd., Mailstop 11, Los 
      Angeles, CA 90027, U.S.A. swu@chla.usc.edu
FAU - Weinberg, Kenneth I
AU  - Weinberg KI
FAU - Joo, Wan Jong
AU  - Joo WJ
FAU - Quinn, John J
AU  - Quinn JJ
FAU - Franklin, Janet
AU  - Franklin J
FAU - Siegel, Stuart E
AU  - Siegel SE
FAU - Gaynon, Paul S
AU  - Gaynon PS
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Pediatr Hematol Oncol
JT  - Journal of pediatric hematology/oncology
JID - 9505928
RN  - 0 (Antigens, CD)
SB  - IM
MH  - Adolescent
MH  - Antigens, CD/blood
MH  - Blast Crisis/pathology
MH  - Bone Marrow Cells/*pathology
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - Female
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics/*pathology
EDAT- 2003/07/09 05:00
MHDA- 2003/08/21 05:00
CRDT- 2003/07/09 05:00
PHST- 2003/07/09 05:00 [pubmed]
PHST- 2003/08/21 05:00 [medline]
PHST- 2003/07/09 05:00 [entrez]
AID - 10.1097/00043426-200307000-00004 [doi]
PST - ppublish
SO  - J Pediatr Hematol Oncol. 2003 Jul;25(7):520-5. doi: 
      10.1097/00043426-200307000-00004.