PMID- 12850102
OWN - NLM
STAT- MEDLINE
DCOM- 20031208
LR  - 20191107
IS  - 0160-4120 (Print)
IS  - 0160-4120 (Linking)
VI  - 29
IP  - 6
DP  - 2003 Sep
TI  - Brominated dioxin-like compounds: in vitro assessment in comparison to classical 
      dioxin-like compounds and other polyaromatic compounds.
PG  - 861-77
AB  - Recently, several countries agreed to adopt the Stockholm convention on 
      persistent organic pollutants (POPs). One future obligation will be to add other 
      POPs as new evidence becomes available. In vitro cell-based bioassays offer a 
      rapid, sensitive, and relatively inexpensive solution to screen possible POP 
      candidates. In the present study, we investigated the aryl hydrocarbon 
      (Ah)-receptor activity of several dioxin-like POPs by using the Micro-EROD 
      (Ethoxy-Resorufin-O-Deethylase) and DR-CALUX (Dioxin-Responsive-Chemical 
      Activated Luciferase gene eXpression) bioassays, which are two state-of-the-art 
      methods. The Micro-EROD system used in our study utilizes a wild-type rat liver 
      cell line (rat liver H4IIEC3/T cells), while the DR-CALUX bioassay consists of a 
      genetically modified rat hepatoma H4IIE cell line that incorporates the firefly 
      luciferase gene coupled to dioxin-responsive elements (DREs) as a reporter gene. 
      In the case of the DR-CALUX bioassay, we used an exposure time of 24 h, whereas 
      we used a 72-h exposure time in the Micro-EROD bioassay. The aim of this study 
      was to compare conventional dioxin-like POPs (such as polychlorinated 
      dibenzodioxins and -furans, PCDD/Fs and coplanar polychlorinated biphenyls, PCBs) 
      with several other classes of possible candidates to be added to the current 
      toxicity equivalent factor (TEF) model in the future. Therefore, this study 
      compares in vitro CYP1A1 (Micro-EROD bioassay) and firefly luciferase induction 
      (DR-CALUX bioassay) in several mixed polyhalogenated dibenzodioxins and -furans 
      (PXDD/Fs; X=Br, Cl, or F), alkyl-substituted polyhalogenated dibenzodioxins and 
      -furans (PMCDD/Fs; M=methyl), polyhalogenated biphenyls (PXBs, X=Br, Cl ), 
      polybrominated diphenyl ethers (PBDEs), pentabromophenols (PBPs), and 
      tetrabromobisphenol-A (TBBP-A). We also evaluate congener-specific relative 
      potencies (REPs) and efficacies (% of TCDD(max)) and discuss the dose-response 
      curves of these compounds, as well as the dioxin-like potency of several other 
      Ah-receptor agonists, such as those of the polyaromatic hydrocarbons (PAHs) and 
      polychlorinated naphthalenes (PCNs). The highest REP values were found for 
      several PXDD/F congeners, followed by some coplanar PXBs, trichlorinated PCDD/Fs, 
      PAHs, PBDE-126, 1-6-HxCN, and some brominated flame retardants (TBBP-A). These in 
      vitro investigations indicate that further research is necessary to evaluate more 
      Ah-receptor agonists for dioxin-like potency.
FAU - Behnisch, Peter Alexander
AU  - Behnisch PA
AD  - Life Science Research Laboratories, Kaneka Corporation, 1-8 Miyamae-Machi, Hyogo 
      Takasago 676-8688, Japan. peterbehnisch@aol.com
FAU - Hosoe, Kazunori
AU  - Hosoe K
FAU - Sakai, Shin-ichi
AU  - Sakai S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Environ Int
JT  - Environment international
JID - 7807270
RN  - 0 (Dioxins)
RN  - 0 (Hydrocarbons, Brominated)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (Receptors, Aryl Hydrocarbon)
SB  - IM
MH  - Animals
MH  - Biological Assay
MH  - Cell Line
MH  - Dioxins/*chemistry
MH  - Dose-Response Relationship, Drug
MH  - Hydrocarbons, Brominated/*chemistry
MH  - In Vitro Techniques
MH  - Polycyclic Aromatic Hydrocarbons/*chemistry
MH  - Rats
MH  - Receptors, Aryl Hydrocarbon/chemistry/*metabolism
EDAT- 2003/07/10 05:00
MHDA- 2003/12/10 05:00
CRDT- 2003/07/10 05:00
PHST- 2003/07/10 05:00 [pubmed]
PHST- 2003/12/10 05:00 [medline]
PHST- 2003/07/10 05:00 [entrez]
AID - S0160412003001053 [pii]
AID - 10.1016/s0160-4120(03)00105-3 [doi]
PST - ppublish
SO  - Environ Int. 2003 Sep;29(6):861-77. doi: 10.1016/s0160-4120(03)00105-3.