PMID- 12850370
OWN - NLM
STAT- MEDLINE
DCOM- 20030812
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 144
IP  - 2
DP  - 2003 Jul 15
TI  - Chromosomal instability in osteosarcoma and its association with centrosome 
      abnormalities.
PG  - 91-9
AB  - The mechanism that generates the extreme aneuploidy that characterizes 
      osteosarcoma (OS) is poorly understood. In this study, interphase fluorescence in 
      situ hybridization (FISH) analysis was used to enumerate cell-to-cell variation 
      of several different chromosomes. We also investigated whether there was an 
      association between TP53 mutation and centrosome aberrations in the generation of 
      chromosomal aneuploidy in OS in four OS cell lines (HOS, SAOS2, U2OS, and MG63) 
      and in a subset of seven tumors. Our analysis showed that there was a wide range 
      of numerical changes affecting multiple chromosomes in OS cell lines and tumors. 
      These data suggest that chromosomal instability (CIN) could be responsible for 
      the extensive aneuploidy associated with this tumor. The results also showed an 
      increased frequency of atypical mitotic figures in three OS cell lines with 
      defective TP53, function and significantly, a more marked CIN phenotype was 
      present in these lines. Furthermore, numerical aberrations of centrosomes were 
      also present in these three OS cell lines with TP53 mutations. In two of three OS 
      patients' tumors there was a large increase in the percentage of abnormal 
      centrosome numbers. We conclude that CIN is a consistent feature of OS and that 
      an intrinsic disturbance of the chromosomal segregation mechanisms is likely 
      associated with centrosome aberrations.
FAU - Al-Romaih, K
AU  - Al-Romaih K
AD  - Department of Laboratory Medicine and Pathobiology, University of Toronto, 
      Toronto, Ontario, Canada.
FAU - Bayani, J
AU  - Bayani J
FAU - Vorobyova, J
AU  - Vorobyova J
FAU - Karaskova, J
AU  - Karaskova J
FAU - Park, P C
AU  - Park PC
FAU - Zielenska, M
AU  - Zielenska M
FAU - Squire, J A
AU  - Squire JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Bone Neoplasms/*genetics
MH  - Centrosome/*pathology
MH  - Child
MH  - *Chromosome Aberrations
MH  - Female
MH  - Genes, p53
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mitosis
MH  - Mutation
MH  - Osteosarcoma/*genetics
MH  - Tumor Cells, Cultured
EDAT- 2003/07/10 05:00
MHDA- 2003/08/13 05:00
CRDT- 2003/07/10 05:00
PHST- 2003/07/10 05:00 [pubmed]
PHST- 2003/08/13 05:00 [medline]
PHST- 2003/07/10 05:00 [entrez]
AID - S0165460802009299 [pii]
AID - 10.1016/s0165-4608(02)00929-9 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2003 Jul 15;144(2):91-9. doi: 
      10.1016/s0165-4608(02)00929-9.