PMID- 12851692
OWN - NLM
STAT- MEDLINE
DCOM- 20040304
LR  - 20061115
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 23
IP  - 2
DP  - 2003 Aug
TI  - Selective up-regulation of tumor necrosis factor receptor I in tumor-bearing rats 
      with cancer-related cachexia.
PG  - 429-36
AB  - Tumor necrosis factor-alpha (TNF-alpha) and interleukin 6 (IL-6) are important 
      mediators in cancer cachexia; however, the expression of these cytokines and 
      their receptors in tumor-bearing animals is poorly characterized. We analyzed 
      expression of TNF-alpha, IL-6, tumor necrosis factor (TNF-RI, TNF-RII) and 
      interleukin 6 (IL-6R) receptors in the brain, kidney, spleen, liver, muscle, 
      ascite tumors and serum, from Yoshida AH-130 hepatoma-bearing rats. TNF-alpha 
      increased in the brain, spleen, liver, and muscle of cachectic animals; IL-6 
      increased in the liver and muscle. AH-130 cells expressed a good level of 
      TNF-alpha; on the contrary, no TNF-alpha or IL-6 protein was detected in the 
      serum of either tumor-bearing or control animals. TNF-RI mRNA was up-regulated in 
      the spleen, liver and muscle of tumor-bearing rats. TNF-RI protein levels 
      confirmed up-regulation in the spleen and liver, but failed to detect any 
      increase in the muscle. Western blotting against TNF-RI revealed two bands of 
      lower molecular weight in cachectic muscle, suggesting proteolysis involving 
      TNF-RI. No significant increase of either TNF-RII or IL-6R was observed. This is 
      the first demonstration of a selective up-regulation of TNF-RI in cancer cachexia 
      and suggests that local production of TNF-alpha and IL-6 is a corner-stone in the 
      induction/maintenance of this syndrome.
FAU - Catalano, Maria G
AU  - Catalano MG
AD  - Laboratorio di Endocrinologia Oncologica, Dipartimenti di Fisiopatologia Clinica 
      and Oncologia, Universita di Torino, I-10126 Turin, Italy.
FAU - Fortunati, Nicoletta
AU  - Fortunati N
FAU - Arena, Katia
AU  - Arena K
FAU - Costelli, Paola
AU  - Costelli P
FAU - Aragno, Manuela
AU  - Aragno M
FAU - Danni, Oliviero
AU  - Danni O
FAU - Boccuzzi, Giuseppe
AU  - Boccuzzi G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Antigens, CD)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (Receptors, Interleukin-7)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type II)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Antigens, CD/genetics/*metabolism
MH  - Cachexia/etiology/*metabolism
MH  - Interleukin-6/metabolism
MH  - Male
MH  - Neoplasms, Experimental/complications/*metabolism/pathology
MH  - RNA, Messenger/genetics
MH  - RNA, Neoplasm/genetics
MH  - Rats
MH  - Receptors, Interleukin-7/metabolism
MH  - Receptors, Tumor Necrosis Factor/genetics/*metabolism
MH  - Receptors, Tumor Necrosis Factor, Type I
MH  - Receptors, Tumor Necrosis Factor, Type II
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Distribution
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Up-Regulation
EDAT- 2003/07/10 05:00
MHDA- 2004/03/05 05:00
CRDT- 2003/07/10 05:00
PHST- 2003/07/10 05:00 [pubmed]
PHST- 2004/03/05 05:00 [medline]
PHST- 2003/07/10 05:00 [entrez]
PST - ppublish
SO  - Int J Oncol. 2003 Aug;23(2):429-36.